Mixtures of 5-hydroxymethylfurfural, levulinic acid, and formic acid have different impact on H-2-producing Clostridium strains

Autor(es)

Giraldeli, Lucas Diniz ^[1] ; Fonseca, Bruna Constante ^[1] ; Reginatto, Valeria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Quim, FFCLRP, Av Bandeirantes 3900, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 43, n. 49, p. 22159-22169, DEC 6 2018.
• Citações Web of Science: 1

Resumo

Fermentative hydrogen production allows the use of renewable biomasses as feedstocks. However, biomass saccharification results not only in carbohydrates, but also in products that can inhibit fermentation. Although biomass hydrolysates contain mixtures of inhibitors, most studies are performed with a single inhibitor. This study evaluates how 5-hydroxymethylfurfural (HMF, 0.60 g/L), levulinic acid (LA, 2.10 g/L), and/or formic acid (FA, 0.80 g/L) mixtures affect two H-2-producing clostridia, Clostridium beijerinckii Br21 and Clostridium acetobutylicum ATCC 824. Fermentation assays with and without (control) the inhibitors helped to calculate the specific H-2-production, substrate consumption, and bacterial cell growth rates for Clostridium beijerinckii Br21 or Clostridium acetobutylicum ATCC 824. HMF + AL, HMF + AF, AL + AF, and HMF + AL + AF mixtures inhibited H-2 production by C. beijerinckii Br21 by 58.7, 60.0, 46.9, and 83.0%, respectively, and by C. acetobutylicum ATCC 824 by 68.1, 71.4, 58.2, and 89.0%, respectively. Clostridium acetobutylicum ATCC 824 metabolized HMF more efficiently. However, organic acids and their combination with HMF inhibited H-2 production by C. beijerinckii Br21 to a lesser extent, which highlighted that this microorganism is robust for H-2 production from biomass hydrolysates. (C) 2018 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 15/06074-1 - Produção de biohidrogênio de terceira geração a partir de biomassa de alga e seus derivados de hidrólise
• Beneficiário: Valeria Reginatto Spiller
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular