Expression profile of a Laccase2 encoding gene during the metamorphic molt in Apis mellifera (Hymenoptera,Apidae)

Autor(es)

Moysés Elias-Neto ^[1] ; Michelle P.M. Soares ^[2] ; Márcia M. G. Bitondi ^[3]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Departamento de Biologia - Brasil

^[2] Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Departamento de Biologia - Brasil

^[3] Universidade de São Paulo. Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Departamento de Biologia - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Revista Brasileira de Entomologia; v. 57, n. 2, p. 213-216, 2013-05-31.

Resumo

Expression profile of a Laccase2 encoding gene during the metamorphic molt in Apis mellifera (Hymenoptera, Apidae). Metamorphosis in holometabolous insects occurs through two subsequent molting cycles: pupation (metamorphic molt) and adult differentiation (imaginal molt). The imaginal molt in Apis mellifera L. was recently investigated in both histological and physiological-molecular approaches. Although the metamorphic molt in this model bee is extremely important to development, it is not well-known yet. In the current study we used this stage as an ontogenetic scenario to investigate the transcriptional profile of the gene Amlac2, which encodes a laccase with an essential role in cuticle differentiation. Amlac2 expression in epidermis was contrasted with the hemolymph titer of ecdysteroid hormones and with the most evident morphological events occurring during cuticle renewal. RT-PCR semiquantitative analyses using integument samples revealed increased levels of Amlac2 transcripts right after apolysis and during the subsequent pharate period, and declining levels near pupal ecdysis. Compared with the expression of a cuticle protein gene, AmelCPR14, these results highlighted the importance of the ecdysteroid-induced apolysis as an ontogenetic marker of gene reactivation in epidermis for cuticle renewal. The obtained results strengthen the comprehension of metamorphosis in Apis mellifera. In addition, we reviewed the literature about the development of A. mellifera, and emphasize the importance of revising the terminology used to describe honey bee molting cycles. (AU)

• Processo FAPESP: 12/09108-6 - Determinação da identidade alar em Blattella germanica(Dictyoptera, Blattellidae)
• Beneficiário: Moysés Elias Neto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/16380-9 - Genética molecular e regulação hormonal da diferenciação do exoesqueleto no inseto-modelo Apis mellifera
• Beneficiário: Marcia Maria Gentile Bitondi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/08300-2 - Diferenciação do adulto em abelhas corbiculadas: heterocronia da morfogênese cuticular e fontes secundárias de ecdisteróides
• Beneficiário: Moysés Elias Neto
• Modalidade de apoio: Bolsas no Brasil - Doutorado