B-mode ultrasonography and ecobiometric parameters for assessment of embryonic and fetal development in sheep

Autor(es):Mostrar menos -

Correia Santos, Victor Jose ^[1] ; Kako Rodriguez, Mariana Garcia ^[1] ; da Silva, Priscila del Aguila ^[1] ; Gomes Mariano, Renata Sitta ^[1] ; Taira, Augusto Ryonosuke ^[1] ; de Almeida, Vivian Tavares ^[2] ; B, Ricardo Andres Ramirez Uscategui ^{[2, 3]} ; Nociti, Ricardo Perecin ^[1] ; Maia Teixeira, Pedro Paulo ^[4] ; Rossi Feliciano, Marcus Antonio ^{[5, 1]} ; Vicente, Wilter Ricardo Russiano ^[1]

Afiliação do(s) autor(es)

^[1] UNESP, Fac Ciencias Agr & Vet, Dept Anim Reprod, Av Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[2] UNESP, Fac Ciencias Agr & Vet, Dept Vet Clin & Surg, Av Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[3] UFVJM, Ave Vereador Joao Narciso 1380, BR-38610000 Unai, MG - Brazil

^[4] Univ Fed Para, Vet Med Coll, Av dos Univ Jaderlandia, BR-68746360 Castanhal, PA - Brazil

^[5] Univ Fed Reconcavo Bahia, Rua Rui Barbosa 710, BR-44380000 Cruz Das Almas, BA - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Animal Reproduction Science; v. 197, p. 193-202, OCT 2018.
• Citações Web of Science: 0

Resumo

The aim in this study was to evaluate the maternal-fetal development during physiological gestation in sheep by means of ultrasonography and to predict the gestational age by mathematical formulae. Thirty Santa Ines sheep were included. Evaluations were performed weekly from the 3rd to 21st gestational week. The Shapiro test was used to determine normality of the data, and Spearman test was used for determining correlations of real or transformed measurements as gestation advanced. If there was a significant difference detected, variable adjustments and gestational weeks were tested using regression models. Significance level was set at 5% and the results presented as mean +/- SD. Principal components analysis (PCA) was used for data clustering and graphic analysis. The gestational vesicle, abdominal, thoracic, biparietal, ocular orbit, and placentome diameters; and the lengths of crown-to-rump, nuchal to snout, scapula, humerus, radius, metacarpus, femur, tibia, metatarsal, heart length, heart width and kidneys were measured. Using pulsed Doppler ultrasonography, heart rate (HR) was obtained. The greatest coefficients of determination (r(2)) were obtained for the biparietal diameter (BPD; 96.5%) and metacarpus length (MCAR; 93%), followed by the abdominal diameter (AD; 91.7%) and heart width (HTW; 90.2%). The PCA analysis indicated graphically that at week 8 to 9, there was no significant change related to technique, whereas, at weeks 19, 20, and 21 a decrease in growth was observed, with the greatest data variability at week 19. Ultrasonographic assessments indicated there were significant correlations between developmental stages of all maternal-fetal structures and the gestational age, which can be used as reference values for gestational age assessments and detection of development abnormalities. (AU)

• Processo FAPESP: 14/15422-0 - Ultrassonografia modo B e Dopplerfluxometria materno fetal na avaliação gestacional de ovelhas
• Beneficiário: Victor José Correia Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado