Preconception immunization can modulate intracellular Th2 cytokine profile in offspring: in vivo influence of interleukin 10 and B/T cell collaboration

Autor(es)

de Oliveira, Marilia Garcia ^[1] ; de Lima Lira, Aline Aparecida ^[1] ; Sgnotto, Fabio Da Ressureicao ^[1] ; Sabo Inoue, Amanda Harumi ^[1] ; da Silva Duarte, Alberto Jose ^{[2, 1]} ; Victor, Jefferson Russo ^{[2, 1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Med Sch, Div Clin Dermatol, Lab Med Invest LIM 56, Av Dr Eneas de Carvalho Aguiar, 500, 3rd Floor, BR-05403000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Med Sch, Div Pathol, Sao Paulo - Brazil

^[3] Laureate Int Univ, FMU, Div Environm Hlth, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CENTRAL EUROPEAN JOURNAL OF IMMUNOLOGY; v. 43, n. 4, p. 378-388, 2018.
• Citações Web of Science: 1

Resumo

Introduction: In the last few years our group has been studying the mechanisms involved in the inhibition of allergy in offspring mediated by preconception maternal immunization, but these mechanisms are not fully understood. Such mechanisms that we have studied aimed at the passive transfer of maternal antibodies and its influence on offspring immune status. Aim of the study: To evaluate whether maternal immunization could modulate intracellular Thl/ Th2 profiles in offspring. Material and methods: C57BL/6 female wild type mice (WT), interleukin (IL)-10(-/-)or CD28(-/-) mice were immunized or not with ovalbumin (OVA) and were mated with respective lineage males and offspring were evaluated at 3 days old (d.o.), 20 d.o., or 20 d.o. after neonatal immunization. Results: Preconception OVA immunization induced a marked reduction in IL-4 secretion by TCD4+ cells of WT offspring when compared with offspring from non-immunized mothers. The maternal immunization of IL-10(-/- )mice induced an increase in the TCD4+IL-4+ percentage in offspring and a reduction in TCD4+IFN-gamma+ cells. The maternal immunization in CD28(-/-) mice induced augment IL-4 intensity in 3 and 20 d.o. offspring TCD4+ cells. Conclusions: Our results reveal that maternal immunization with OVA can down-regulate the Th2 pattern in offspring and this regulation is dependent on IL-10 and B/T cell collaboration. (AU)

• Processo FAPESP: 15/17256-3 - "Investigação dos mecanismos de inibição da resposta de hipersensibilidade tipo I da prole mediada pela imunização materna murina: modulação de citocinas, indução de linfócitos T e B reguladores e influência da natureza do alérgeno."
• Beneficiário: Alberto José da Silva Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/05181-7 - Avaliação translacional do efeito modulador e/ou regulador dos anticorpos IgG na maturação de linfócitos TCD4, TCD8, nTreg, Tgammadelta e B intra-tímicos de neonatos.
• Beneficiário: Alberto José da Silva Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Regular