Determination of amino acids in gym supplements using digital images and paper platform coupled to diffuse reflectance spectroscopy and USB device

Autor(es)

Catelani, Tiago Augusto ^[1] ; Bittar, Dayana Borges ^[1] ; Pezza, Leonardo ^[1] ; Pezza, Helena Redigolo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Quim, UNESP, R Prof Francisco Degni 55, POB 355, BR-24800900 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 196, p. 523-529, MAY 1 2019.
• Citações Web of Science: 2

Resumo

New and reliable methodologies are described for the determination of amino acids in gym supplements, offering rapid and clean analysis, with low generation of waste. The proposed methods are based on the reaction between ninhydrin and amino acids in acetate buffer medium (pH 4.75). Experimental design tools were used to optimize the analytical conditions. The linear range obtained for both methodologies was 20.0-350.0 mg L-1. The detection (LOD) and quantification (LOQ) limits were 6.2 and 21.0 mg L-1, respectively, for diffuse reflectance using a paper filter with hydrophobic barriers to increase the sensitivity and homogeneity of the colored product, and 5.7 and 18.8 mg L-1, respectively, for digital image analysis. A USB device was used in the diffuse reflectance spectroscopy method for heating the filter paper, providing speed and portability for the developed methodology. The methods showed good results when applied to gym supplement samples, with recoveries in the range 93.1-110%. (AU)

• Processo FAPESP: 15/21733-1 - Desenvolvimento de métodos analíticos de vanguarda utilizando spot test e dispositivo microfluidico em papel.
• Beneficiário: Helena Redigolo Pezza
• Modalidade de apoio: Auxílio à Pesquisa - Regular