Biomass Accumulation and Cell Wall Structure of Rice Plants Overexpressing a Dirigent-Jacalin of Sugarcane (ShDJ) Under Varying Conditions of Water Availability

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Andrade, Larissa Mara ^{[1, 2]} ; Peixoto-Junior, Rafael Favero ^{[1, 2]} ; Ribeiro, Rafael Vasconcelos ^[3] ; Nobile, Paula Macedo ^[1] ; Brito, Michael Santos ^{[4, 1]} ; Ribeiro Marchiori, Paulo Eduardo ^{[5, 6]} ; Carlin, Samira Domingues ^[1] ; Boer Martins, Alexandre Palma ^{[1, 2]} ; Goldman, Maria Helena S. ^[7] ; Portilla Llerena, Juan Pablo ^[3] ; Fregonesi, Caroline ^[1] ; Perecin, Dilermando ^[8] ; Carlos de Oliveira Nebo, Joao Felipe ^[9] ; Figueira, Antonio ^[9] ; Benatti, Thiago Romanos ^[10] ; da Silva, Jorge ^[10] ; Mazzafera, Paulo ^[3] ; Creste, Silvana ^[1]

Afiliação do(s) autor(es)

^[1] Ctr Cana, Inst Agron IAC, Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, PPG Genet, Ribeirao Preto - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Plant Biol, Campinas, SP - Brazil

^[4] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, Sao Jose Dos Campos - Brazil

^[5] Univ Fed Lavras, Dept Biol, Lavras - Brazil

^[6] Ctr Ecofisiol & Biofis, Inst Agron IAC, Campinas, SP - Brazil

^[7] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ribeirao Preto - Brazil

^[8] Univ Estadual Paulista Julio de Mesquita Filho Un, Fac Ciencias Agr & Vet, Jaboticabal - Brazil

^[9] Univ Sao Paulo, CENA, Piracicaba - Brazil

^[10] Texas A&M Agrilife Res & Extens Ctr, Weslaco, TX - USA

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PLANT SCIENCE; v. 10, FEB 13 2019.
• Citações Web of Science: 0

Resumo

A sugarcane gene encoding a dirigent-jacalin, ShDJ, was induced under drought stress. To elucidate its biological function, we integrated a ShDJ-overexpression construction into the rice Nipponbare genome via Agrobacterium- mediated transformation. Two transgenic lines with a single copy gene in T-0 were selected and evaluated in both the T-1 and T-4 generations. Transgenic lines had drastically improved survival rate under water deficit conditions, at rates close to 100%, while WT did not survive. Besides, transgenic lines had improved biomass production and higher tillering under water deficit conditions compared with WT plants. Reduced pectin and hemicellulose contents were observed in transgenic lines compared with wild-type plants under both well-watered and water deficit conditions, whereas cellulose content was unchanged in line \#17 and reduced in line \#29 under conditions of low water availability. Changes in lignin content under water deficit were only observed in line \#17. However, improvements in saccharification were found in both transgenic lines along with changes in the expression of OsNTS1/2 and OsMYB58/63 secondary cell wall biosynthesis genes. ShDJ-overexpression upregulated the expression of the OsbZIP23, OsGRAS23, OsP5CS, and OsLea3 genes in rice stems under well-watered conditions. Taken together, our data suggest that ShDJ has the potential for improving drought tolerance, plant biomass accumulation, and saccharification efficiency. (AU)

• Processo FAPESP: 17/24420-0 - Cana transgênica: endereçando o aumento da produtividade sustentável
• Beneficiário: Silvana Aparecida Creste Dias de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 08/57495-3 - Integrando características fisiológicas, morfológicas e anatômicas para entender o rendimento diferencial de sacarose em genótipos de cana-de-açúcar
• Beneficiário: Eduardo Caruso Machado
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 11/50661-8 - Caracterização de transcritos de cana-de-açúcar que respondem ao estresse hídrico visando à obtenção de transgênicos tolerantes à seca
• Beneficiário: Silvana Aparecida Creste Dias de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular