Effect of low power laser in biomodulation of cultured osteoblastic cells of Wistar rats

Autor(es)

Misael da Silva Morsoleto, Maria Jose ^[1] ; Sella, Valeria ^[1] ; Machado, Paula ^[1] ; do Bomfim, Fernando ^[1] ; Fernandes, Maria Helena ^[2] ; Morgado, Fernando ^[3] ; Lopes Filho, Gaspar De Jesus ^[4] ; Plapler, Helio ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Postgrad Program Interdisciplinary Surg Sci, Sao Paulo, SP - Brazil

^[2] Univ Porto, Fac Med Dent, Dept Pharmacol & Cellular Compatibil, Porto - Portugal

^[3] Univ Aveiro, Dept Biol, Aveiro - Portugal

^[4] Univ Fed Sao Paulo, Dept Surg, Sch Med, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Acta Cirurgica Brasileira; v. 34, n. 2 2019.
• Citações Web of Science: 0

Resumo

Abstract Purpose: To analyze aspects of the biomodulating effect of light in biological tissues, bone cells from surgical explants of the femur of rats were irradiated with low intensity laser. Methods: Bone cells were cultured and irradiated with LASER light (GaAlAs). Growth, cell viability, mineralized matrix formation, total protein dosage, immunostimulatory properties, cytochemical analysis, gene expression of bone proteins were examined using live cell imaging and cell counting by colorimetric assay. The gene expression of: alkaline phosphatase (ALP), type 1 collagen, osteocalcin and osteopontin through the real-time polymerase chain reaction. Results: At 8 days, the viability of the irradiated culture was 82.3% and 72.4% in non-irradiated cells. At 18 days, the cellular viability (with laser) was 77.42% and 47.62% without laser. At 8 days, the total protein concentration was 21.622 mg / mol in the irradiated group and 16, 604 mg / mol in the non-irradiated group and at 18 days the concentration was 37.25 mg / mol in the irradiated group and 24, 95 mg / mol in the non-irradiated group. Conclusion: The laser interfered in the histochemical reaction, cell viability, matrix mineralization, and maintained the cellular expression of proteins (AU)

• Processo FAPESP: 10/15551-4 - Efeito in vitro do laser de baixa intensidade na reparação óssea de um modelo de fratura em fêmur de ratos: marcadores de crescimento
• Beneficiário: Helio Plapler
• Modalidade de apoio: Auxílio à Pesquisa - Regular