Dose-response effect of chlorhexidine on a multispecies oral biofilm formed on pure titanium and on a titanium-zirconium alloy

Autor(es)

Silva Souza, Joao Gabriel ^[1] ; Lima, Carolina Veloso ^[2] ; Costa Oliveira, Barbara Emanoele ^[2] ; Ricomini-Filho, Antonio Pedro ^[2] ; Faveri, Marcelo ^[3] ; Sukotjo, Cortino ^[4] ; Feres, Magda ^{[5, 6]} ; Del Bel Cury, Altair Antoninha ^[1] ; Ricardo Barao, Valentim Adelino ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Piracicaba, SP - Brazil

^[2] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Physiol Sci, Piracicaba, SP - Brazil

^[3] Univ Sao Judas Tadeu, Dental Res Div, Sao Paulo - Brazil

^[4] Univ Illinois, Dept Restorat Dent, Chicago, IL - USA

^[5] Univ Sao Judas Tadeu, Sao Paulo - Brazil

^[6] Univ Guarulhos, Dental Res Div, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOFOULING; v. 34, n. 10, p. 1175-1184, 2018.
• Citações Web of Science: 1

Resumo

This study aimed to test the dose-response effect of chlorhexidine on multispecies biofilms formed on commercially pure titanium (cpTi) and titanium-zirconium (TiZr) alloy. Biofilms were formed on cpTi and TiZr discs and treated two times per day with five different chlorhexidine concentrations (0.12, 0.20, 0.50, 1, 2%). The biofilms were collected for microbiological, biochemical and microscopic analyses. The significance of differences among groups was evaluated by linear regression, ANOVA, Bonferroni and Tukey tests. The mean number of colony-forming units decreased as the chlorhexidine concentration increased for both cpTi and TiZr (p<0.05). The maximum effect was observed with the 0.5% concentration. Confocal microscopy images suggested an increase in the number of dead bacterial cells with increased chlorhexidine concentration. The biofilm pH increased after chlorhexidine exposure (p<0.05). Chlorhexidine showed an antimicrobial dose-response effect in controlling biofilm on cpTi and TiZr. 0.5% chlorhexidine can be used to achieve the maximum antimicrobial effect on both materials. (AU)

• Processo FAPESP: 15/23118-2 - Efeito antibacteriano e avaliação da biocompatibilidade do tratamento com plasma de descarga incandescente na superfície do titânio: estudo in vitro e in situ
• Beneficiário: João Gabriel Silva Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado