Oestradiol acts through its beta receptor to increase vasopressin neuronal activation and secretion induced by dehydration

Autor(es)

Vilhena-Franco, Tatiane ^[1] ; Mecawi, Andre Souza ^[2] ; Almeida-Pereira, Gislaine ^[1] ; Lucio-Oliveira, Fabiana ^[3] ; Kagohara Elias, Lucila Leico ^[1] ; Antunes-Rodrigues, Jose ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Physiol, Ribeirao Preto Med Sch, Ribeirao Preto - Brazil

^[2] Univ Fed Sao Paulo, Dept Biophys, Paulista Sch Med, Sao Paulo - Brazil

^[3] Sci & Technol Southern Minas Gerais, Fed Inst Educ, Muzambinho - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Neuroendocrinology; v. 31, n. 4 APR 2019.
• Citações Web of Science: 0

Resumo

Vasopressinergic neurones of the supraoptic (SON) and paraventricular (PVN) nuclei express oestrogen receptor (ER)beta and receive afferent projections from osmosensitive neurones that express ER alpha. However, which subtype of these receptors mediates the effects of oestradiol on vasopressin (AVP) secretion induced by hydromineral challenge has not yet been demonstrated in vivo. Moreover, AVP secretion induced by hyperosmolality is known to involve activation of TRPV1 (transient receptor potential vanilloid, member 1) in magnocellular neurones, although whether oestradiol modulates expression of this receptor is unknown. Thus, the present study aimed to clarify the mechanisms involved in the modulation exerted by oestradiol on AVP secretion, specifically investigating the involvement of ER beta, ER alpha and TRPV1 receptors in response to water deprivation (WD). We observed that treatment with an ER beta agonist potentiated AVP secretion and vasopressinergic neuronal activation induced by WD. This increase in AVP secretion induced by WD was reversed by an ER beta antagonist. By contrast to ER beta, the ER alpha agonist did not alter plasma AVP concentrations or activation of AVP neurones in the SON and PVN. Additionally, Fos expression in the subfornical organ was not altered by the ER alpha agonist. TRPV1 mRNA expression was increased by WD in the SON, although this response was not altered by any treatment. The results of the present study suggest that ER beta mediates the effects of oestradiol on AVP secretion in response to WD, indicating that the effects of oestradiol occur directly in AVP neurones without affecting TRPV1. (AU)

• Processo FAPESP: 14/15218-4 - Regulação da homeostase energética e do balanço hidromineral: das células aos sistemas fisiológicos
• Beneficiário: Tatiane Vilhena Franco
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado