Sequential Enzymatic and Mild-Acid Hydrolysis of By-Product of Carrageenan Process from Kappaphycus alvarezii

Autor(es)

Paz-Cedeno, Fernando Roberto ^[1] ; Solorzano-Chavez, Eddyn Gabriel ^[1] ; de Oliveira, Levi Ezequiel ^[2] ; Gelli, Valeria Cress ^[3] ; Monti, Rubens ^[4] ; de Oliveira, Samuel Conceicao ^[1] ; Masarin, Fernando ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Sao Paulo State Univ, Sch Pharmaceut Sci FCF, Dept Bioproc & Biotechnol, BR-14800903 Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Lorena Sch Engn EEL, Dept Chem Engn, Lorena, SP - Brazil

^[3] Fisheries Inst IP, North Coast Res & Dev Ctr, Secretariat Agr & Supply State Sao Paulo, Sao Paulo - Brazil

^[4] UNESP Sao Paulo State Univ, Sch Pharmaceut Sci FCF, Dept Food & Nutr, BR-14800903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BioEnergy Research; v. 12, n. 2, p. 419-432, JUN 2019.
• Citações Web of Science: 2

Resumo

Kappaphycus alvarezii is a red macroalgae widely used to produce carrageenan. The carrageenan processing produces a by-product rich in glucan which has been reported as easily hydrolyzed with enzymes, but the hydrolysate forms a gel at usual fermentation temperatures. The purpose of this study was to evaluate the enzymatic hydrolysis integrated with a mild-acid treatment of the by-product to obtain a hydrolysate rich in monomeric sugars. Using an enzyme load of 10FPUg(-1) of by-product, close to 100 and 14.7% of glucan and galactan conversion were reached, respectively. Increasing the enzyme load to 100FPUg(-1) raised the galactan conversion to 30%. The mild-acid treatment after enzymatic hydrolysis was satisfactory, increasing the glucose and galactose concentrations, without producing significant amounts of fermentation inhibitors and avoiding the formation of a gel structure. The statistical analysis showed that the main effects on the response were negative for the three independent variables, meaning that the selectivity (S) becomes lower when experimental conditions at the higher levels are used (longer time, higher temperature, and acid concentration). Therefore, the integrated enzymatic and acid hydrolysis of the by-product becomes a promising technological route to produce monomeric sugars for bioethanol or fine chemical production. (AU)

• Processo FAPESP: 14/05969-2 - Caracterização química e sacarificação de polissacarídeos de diferentes cepas da macroalga Kappaphycus alvarezii
• Beneficiário: Fernando Masarin
• Modalidade de apoio: Auxílio à Pesquisa - Regular