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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Establishment of 3-dimensional scaffolds from hemochorial placentas

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Autor(es):
Favaron, Phelipe O. [1] ; Borghesi, Jessica [1] ; Mess, Andrea Maria [1] ; Castelucci, Patricia [2] ; Matias, Gustavo de Sa Schiavo [1] ; Nunes Barreto, Rodrigo da Silva [1] ; Miglino, Maria Angelica [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Surg, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Sao Paulo, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Placenta; v. 81, p. 32-41, JUN 2019.
Citações Web of Science: 0
Resumo

Introduction: The extracellular matrix (ECM) is a complex, tissue-specific 3-dimensional network that controls cell processes. ECMs derived from various organs are used to produce biological scaffolds comparable to the native microenvironment. Although placentas are often overlooked, they offer a rich ECM for tissue engineering, especially the hemochorial placentas from rodents and lagomorphs that resemble the ones from humans. Methods: Here we established a protocol for decellularization and investigated the ECM in native and decellularized placentas of guinea pigs, rats and rabbits by means of histology, immunohistochemistry, immunofluorescence and scanning electron microscopy. Results: Effective decellularization were achieved by immersion in 0.25% Sodium Dodecyl Sulfate for 3 days, resulting in an intact ECM, while cells or nuclei were absent. All species had a high diversity of ECM components that varied between areas. Discussion: Dense fibrous networks in the junctional zone were strongly positive to collagen I, III and IV, fibronectin, and laminin ECM markers. Noticeable response were also found for the decidua, especially along the maternal vessels. The labyrinth had thin fibers strongly positive for fibronectin and laminin, but not much for collagens. In conclusion, we established an effective protocol to obtain biological scaffolds from animal models with hemochorial placentas that possessed promising values for future purposes in Regenerative Medicine. (AU)

Processo FAPESP: 14/50844-3 - Matriz extracelular na saúde e matriz placentária na regeneração de tecidos
Beneficiário:Maria Angelica Miglino
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/22350-9 - Matriz extracelular na placentação de diferentes espécies de mamíferos
Beneficiário:Maria Angelica Miglino
Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional