| Texto completo | |
| Autor(es): |
Buarque de Gusmao, Carlos Vinicius
[1]
;
Batista, Nilza Alzira
[1]
;
Vidotto Lemes, Valeria Trombini
[1]
;
Maia Neto, Wilson Leite
[1]
;
de Faria, Lidia Dornelas
[1]
;
Alves, Jose Marcos
[2]
;
Belangero, William Dias
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] State Univ Campinas UNICAMP, Fac Med Sci, Dept Orthoped & Traumatol, Campinas, SP - Brazil
[2] Univ Sao Paulo, Coll Engn Sao Carlos, Elect Engn Dept, Sao Carlos, SP - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ULTRASOUND IN MEDICINE AND BIOLOGY; v. 45, n. 8, p. 2140-2161, AUG 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
An experimental study was conducted to determine whether low-intensity pulsed ultrasound stimulation (LIPUS), extracorporeal shockwave treatment (ESWT) and radial pressure wave treatment (RPWT) modulate Akt, bone morphogenetic protein-2 (BMP-2), extracellular signal-regulated kinase-2 (ERK-2), focal adhesion kinase (FAK) and transforming growth factor-beta 1 (TGF-beta 1) during bone healing in rat tibial defects. Rat tibial defects were exposed to 500 shots of ESWT delivered at 0.12 mJ/mm(2), 500 impulses of RPWT operated at 2.0 bar or to daily 20-min 30 mW/cm(2) LIPUS. Following 1, 3 and 6 wk, bones were harvested to determine the expression and activity of Akt, BMP-2, ERK-2, FAK and TGF-beta 1. Animals exposed to ultrasound were followed up to 3 wk. Protein expression and activity were unchanged following LIPUS treatment. ESWT increased Akt activity 2.11-fold (p = 0.043) and TGF-beta 1 expression 9.11-fold (p = 0.016) at 1 wk and increased FAK activity 2.16-fold (p = 0.047) at 3 wk. RPWT increased FAK activity 2.6-fold (p = 0.028) at 3 wk and decreased Akt expression 0.52-fold (p = 0.05) at 6 wk. In conclusion, the protocols employed for ESWT and RPWT modulated distinct signaling pathways during fracture healing, while LIPUS standard protocol did not change the usual signaling pathways of the proteins investigated. Future studies are required to monitor osteogenesis so that the biologic meaning of our results can be clarified. (C) 2019 World Federation for Ultrasound in Medicine \& Biology. All rights reserved. (AU) | |
| Processo FAPESP: | 14/26729-0 - Estudo biomecânico e biomolecular de métodos terapêuticos de regeneração óssea |
| Beneficiário: | William Dias Belangero |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |