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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Dual effective core-shell electrospun scaffolds: Promoting osteoblast maturation and reducing bacteria activity

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Autor(es):
Machado De-Paula, Mirian Michelle [1] ; Afewerki, Samson [2, 3] ; Viana, Bartolomeu Cruz [4, 5] ; Webster, Thomas Jay [6] ; Lobo, Anderson Oliveira [7] ; Marciano, Fernanda Roberta [4, 5]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Vale Paraiba, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[2] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 - USA
[3] Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Div Engn Med, Cambridge, MA 02139 - USA
[4] Univ Fed Piaui, Dept Phys, BR-64049550 Teresina, PI - Brazil
[5] Univ Fed Piaui, Mat Sci & Engn Grad Program, BR-64049550 Teresina, PI - Brazil
[6] Northeastern Univ, Dept Chem Engn, Nanomed Lab, Boston, MA 02115 - USA
[7] Univ Fed Piaui, Dept Mat Engn, Interdisciplinary Lab Adv Mat, BR-64049550 Teresina, PI - Brazil
Número total de Afiliações: 7
Tipo de documento: Artigo Científico
Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 103, OCT 2019.
Citações Web of Science: 0
Resumo

Herein, we electrospun ultrathin core-shell fibers based on polycaprolactone (PCL), polyethylene glycol (PEG), gelatin and osteogenic growth peptide (OGP), and evaluated their potential to upregulate human osteoblast cells (hFOB) and to reduce Gram-positive and Gram-negative bacteria. We also evaluated the fiber morphology, chemical structure and peptide delivery efficacy. The employment of core-shell fibers compared to fibers without a core-shell showed improved mechanical strength, comparable to the strength of pure PCL, as well as improved hydrophilicity and wettability. The careful selection of polymer combination and core-shell strategy promoted a controlled and sustained release of OGP. Moreover, increased calcium deposition (CD) (1.3-fold) and alkaline phosphate (ALP) activity was observed when hFOBs were cultivated onto core-shell fibers loaded with OGP after 21 days of culture. Our developed scaffolds were also able to reduce the amount of Pseudomonas aeruginosa (ATCC 25668) bacteria by a factor of two compared to raw PCL without the use of any antibiotics. All of these results demonstrate a promising potential of the developed core-shell electrospun scaffolds based on PCL:PEG:Gelatin:OGP for numerous bone tissue applications. (AU)

Processo FAPESP: 14/16295-2 - Nanofibras produzidos por eletrofiação e rotofiação com nanopartículas de hidroxiapatita e nanotubos de carbono incorporados para verificar sua potencialidade na regeneração óssea
Beneficiário:Mirian Michelle Machado de Paula
Linha de fomento: Bolsas no Brasil - Doutorado