Influence of Green Tea Extract with Different Concentrations of Epigallocatechin Gallate on Calvaria Bone Repair of Ovariectomized Rats

Autor(es):Mostrar menos -

Luana Cristina de Freitas ^[1] ; Luiz Gustavo de Sousa ^[2] ; Guilherme Luciano Leite ^[3] ; Priscilla Hakime Scalize ^[4] ; Dimitrius Leonardo Pitol ^[5] ; Mariah Acioli Righetti ^[6] ; Vitória Ricardo ^[7] ; Edneia Corrêa de Mello ^[8] ; Karina Fittipaldi Bombonato-Prado ^[9] ; Simone Cecilio Hallak Regalo ^[10] ; Selma Siessere ^[11]

Afiliação do(s) autor(es):Mostrar menos -

^[1] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Basic and Oral Biology - Brasil

^[2] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Basic and Oral Biology - Brasil

^[3] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Basic and Oral Biology - Brasil

^[4] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Basic and Oral Biology - Brasil

^[5] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Basic and Oral Biology - Brasil

^[6] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Basic and Oral Biology - Brasil

^[7] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Basic and Oral Biology - Brasil

^[8] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Basic and Oral Biology - Brasil

^[9] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Basic and Oral Biology - Brasil

^[10] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Basic and Oral Biology - Brasil

^[11] University of São Paulo. School of Dentistry of Ribeirão Preto. Department of Basic and Oral Biology - Brasil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MORPHOLOGY; v. 37, n. 4, p. 1325-1330, 2019-12-00.

Resumo

SUMMARY: Impairing osteoporosis progression is a challenge, and recently the role of antioxidants has been associated to bone metabolism. Green tea extract is rich in catechins, especially epigallocatechin gallate (EGCG), which may help control osteoporosis damage in bone tissue. This investigation evaluated the efficacy of green tea ingestion containing different concentrations of EGCG in calvaria bone repair of ovariectomized rats. Wistar rats (n=15) were ovariectomized and divided into 3 groups: ovariectomized (OVX), ovariectomized + GTE 15 % EGCG (OVX/GTE15), and ovariectomized + GTE 94 % EGCG (OVX/GTE94). Green tea extract was administered by gavage in the concentration of 50 mg/kg and sham group (n=5) received water. Bone defects were performed in the calvaria 60 days after ovariectomy followed by 4 weeks until euthanasia. Bone samples were collected to perform qualitative and quantitative histological analysis of bone formation. Data obtained were submitted to normality and ANOVA statistical test for p<0.05. The mean values of neoformed bone for Sham, OVX, OVX/GTE15 and OVX/GTE94 were respectively: 21.11 ± 3.91; 19.92 ± 2.20; 33.05 ± 1.26 e 34.75 ± 0.54 (p<0.05). Results show that continuous ingestion of green tea extract immediately after ovariectomy shows positive effects in the prevention of bone loss in osteoporosis, even with low concentrations of EGCG. (AU)

• Processo FAPESP: 16/08720-0 - Efeito do extrato de chá verde sobre o reparo ósseo em ratas ovariectomizadas
• Beneficiário: Luana Cristina de Freitas
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica