Ultraviolet Light Treatment of Titanium Suppresses Human Ora Bacterial Attachment and Biofilm Formation: A Short-Term In Vitro Study

Autor(es)

Ishijima, Manabu ^[1] ; de Avila, Erica Dorigatti ^{[2, 3]} ; Nakhaei, Kourosh ^[1] ; Shi, Wenyuan ^{[4, 2]} ; Lux, Renate ^{[2, 5]} ; Ogawa, Takahiro ^[1]

Afiliação do(s) autor(es)

^[1] Univ Calif Los Angeles, Sch Dent, Div Adv Prosthodont, Weintraub Ctr Reconstruct Biotechnol, 10833 Le Conte Ave, B3-081 CHS, Box 951668, Los Angeles, CA 90095 - USA

^[2] Univ Calif Los Angeles, Sch Dent, Div Oral Biol & Med, Los Angeles, CA 90095 - USA

^[3] Sao Paulo State Univ, UNESP, Sch Dent Araraquara, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

^[4] Forsyth Inst, Cambridge, MA - USA

^[5] Univ Calif Los Angeles, Sch Dent, Div Constitut & Regenerat Sci, Los Angeles, CA 90095 - USA

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS; v. 34, n. 5, p. 1100-1108, SEP-OCT 2019.
• Citações Web of Science: 0

Resumo

Purpose: Antibacterial dental implants and related prosthetic components could help to reduce infection and prevent peri-implantitis. The purpose of this study was to determine the effect of ultraviolet (UV) light treatment of titanium on biofilm formation of human oral bacteria. Materials and Methods: Machine-prepared commercially pure titanium disks were treated with UV light for 12 minutes. Human oral bacteria were seeded onto untreated and UV-treated disks. Early bacterial attachment to titanium was assessed at 12 hours. Surface topography of initial biofilms was evaluated by 3D scanning electron microscopy at 24 hours. The quantity and morphology of subsequent colony development and bioflim formation were examined by confocal laser scanning microscopy for up to 7 days. Results: Throughout the time course, significantly fewer bacterial cells attached to UV-treated titanium surfaces compared with untreated ones. While biofilm developed rapidly to a final thickness of approximately 16 mu m by day 3 on untreated titanium, on UV-treated surfaces it remained below 8 mu m, even at day 7. Similarly, UV treatment resulted in 70% less exopolysaccharide (EPS) volume than on untreated surfaces at day 7. This is consistent with the finding that EPS production per cell was significantly lower on UV-treated surfaces. Untreated titanium surfaces covered with bioflim were fivefold rougher than the original machined surface, while UV-treated surfaces remained twofold rougher due to significantly less biofilm formation. Conclusion: UV treatment of titanium surfaces significantly reduces attachment of human oral bacteria and subsequent biofilm formation as well as EPS production for at least 7 days. UV treatment prevented the escalation of surface colonization, mitigating an unfavorable bacteriophilic cascade and environmental trigger for biofilm formation. (AU)

• Processo FAPESP: 11/05106-6 - Estudo sobre a arquitetura e o comportamento dos biofilmes simples e misto - Fusobacterium nucleatum e Porphyromonas gingivalis - frente a duas diferentes superfícies de abutments de implantes
• Beneficiário: Érica Dorigatti de Avila
• Modalidade de apoio: Bolsas no Brasil - Mestrado