Antimicrobial Activity and Physicochemical Properties of Antibiotic Pastes Used In Regenerative Endodontics

Autor(es)

Rafaela Fernandes Zancan ^[1] ; Bruno Cavalini Cavenago ^[2] ; Denise Ferracioli Oda ^[3] ; Clovis Monteiro Bramante ^[4] ; Flaviana Bombarda de Andrade ^[5] ; Marco Antonio Hungaro Duarte ^[6]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. Bauru Dental School. Department of Restorative Dentistry, Dental Materials and Endodontics - Brasil

^[2] Universidade Federal do Paraná. Department of Restorative Dentistry - Brasil

^[3] Universidade de São Paulo. Bauru Dental School. Department of Restorative Dentistry, Dental Materials and Endodontics - Brasil

^[4] Universidade de São Paulo. Bauru Dental School. Department of Restorative Dentistry, Dental Materials and Endodontics - Brasil

^[5] Universidade de São Paulo. Bauru Dental School. Department of Restorative Dentistry, Dental Materials and Endodontics - Brasil

^[6] Universidade de São Paulo. Bauru Dental School. Department of Restorative Dentistry, Dental Materials and Endodontics - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 30, n. 6, p. 536-541, 2019-11-28.

Resumo

Abstract: The purpose of this study was to evaluate the pH, solubility and antimicrobial action of Calcium Hydroxide Paste (CH), Double Antibiotic Paste (metronidazole+ciprofloxacin-DAP), calcium hydroxide added to DAP (CH/DAP) and Triple Antibiotic Paste (metronidazole + ciprofloxacin+minocycline-TAP). pH (n=10) were measured by pHmeter. Root canals of acrylic teeth (n=10) were filled with the above-mentioned intracanal-dressings, immersed in ultrapure water, and solubility was measured by the difference between the initial and final volume (7,15 and 30 days) by using micro-computed tomography. Enterococcus faecalis biofilm was induced on bovine dentin disc surfaces (n=20), and treated with the pastes for 7 days. Percentage bacterial viability was verified by confocal microscope, with LIVE/DEAD dye. CH and CH/DAP presented the highest pH values. Regarding solubility, after 7 days, antibiotic groups presented significant volume loss. CH and CH/DAP showed no statistical difference compared with the Control in antimicrobial action against E. faecalis biofilm. However, TAP and DAP presented a significant percentage reduction in bacterial population. Due to high solubility of the pastes, renewing antibiotic dressings every 7 days, or using the medications for this period in regeneration protocols is recommended. DAP is indicated for killing E. faecalis in biofilm because it has antimicrobial action similar to TAP. Adding Calcium Hydroxide to DAP significantly decreased its antimicrobial action. In spite of its the low solubility and high pH values, the CH paste showed a low level of antimicrobial action against E. faecalis in biofilm. (AU)

• Processo FAPESP: 14/17473-1 - Ação antimicrobiana e propriedades físico-químicas de diferentes pastas empregadas como medicação intracanal
• Beneficiário: Rafaela Fernandes Zancan
• Modalidade de apoio: Bolsas no Brasil - Mestrado