| Texto completo | |
| Autor(es): |
de Campos da Costa, Joao Paulo
[1]
;
Bastos, Wagner Benicio
[2]
;
da Costa, Paulo Inacio
[3]
;
Zaghete, Maria Aparecida
[4]
;
Longo, Elson
[2]
;
Carmo, Joao Paulo
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Elect Engn SEL, BR-13566590 Sao Carlos, SP - Brazil
[2] Fed Univ Sao Carlos UFSCar, Dept Chem, INCTMN CDMF, BR-13565905 Sao Carlos, SP - Brazil
[3] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Clin Anal, Lab Clin Immunol & Mol Biol LICBM, BR-14800903 Araraquara, SP - Brazil
[4] Sao Paulo State Univ UNESP, Inst Chem, BR-14800903 Araraquara, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | IEEE SENSORS JOURNAL; v. 19, n. 22, p. 10701-10709, NOV 15 2019. |
| Citações Web of Science: | 0 |
| Resumo | |
This paper presents a portable laboratory platform (PLP) with electrochemical amperometric biosensors for the detection of hepatitis C virus (HCV). This PLP is composed by a controller unit, a Bluetooth module to provide communication with mobile devices, and a potentiostat circuit to perform cyclic voltammetry. The Bluetooth communication allows the mobile devices to set up the parameters, e. g., a cycling voltage in the range +/- 1.5 V, a scan rate in the range 5-1000 mV(-1) with a step potential of 2.318 mV, and up to 4000 number of scan cycles. The electrochemical sensors were functionalized with cystamine (C4H12N2S2) and glutaraldehyde (C5H8O2) to make the covalent binding with the recombinant core-HCV protein. The experimental results have revealed a positive detection of HCV, using a redox solution of 5 mmol.L-1 of potassium ferrocyanide (K-3 Fe(CN)(6)) on 0.1 mol.L-1 of phosphate-buffered saline (PBS). The sensitivity of the immunosensor was established at the concentration of 1 ng.mu L-1 of the anti-core-HCV IgG class antibody. This PLP has a plug-and-play connection for the biosensors, and at the same time, it offers good ergonomy with easy operation due to its low dimensions (115 mm x 75 mm x 30 mm). This PLP presents a power consumption of 280.5 mW when supplied with a 3.3-V voltage source. This consumption was measured in the worst operation case, e.g., with the Bluetooth working, resulting in an average battery life of 195 min. (AU) | |
| Processo FAPESP: | 17/10819-8 - CDMF - Centro de Desenvolvimento de Materiais Funcionais |
| Beneficiário: | Wagner Benício Bastos |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais |
| Beneficiário: | Elson Longo da Silva |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |