A simple and effective approach to produce tubular polysaccharide-based hydrogel scaffolds

Autor(es)

Bombaldi de Souza, Fernanda Carla ^[1] ; Cannasao, Dimitria Bonizol ^[2] ; Bombaldi de Souza, Renata Francielle ^[1] ; Drouin, Bernard ^[2] ; Mantovani, Diego ^[2] ; Moraes, Angela Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Chem Engn, Dept Engn Mat & Bioproc, UNICAMP, Campinas, SP - Brazil

^[2] Laval Univ, Lab Biomat & Bioengn, Canada Res Chair Biomat & Bioengn Innovat Surg 1, Dept Min Met Mat Engn, Res Ctr, CHU Quebec, Div Rege, Quebec City, PQ - Canada

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Polymer Science; v. 137, n. 13 APR 5 2020.
• Citações Web of Science: 2

Resumo

The production of porous tubular scaffolds is of great interest in the field of tissue engineering, given the existence of several tubular structures in the human body. In this work, a methodology was developed for the fabrication of tubular-shaped scaffolds based on the casting of polymeric solutions by controlled crosslinking mediated by a semipermeable cast. The fabrication of hydrogel tubular scaffolds from chitosan-pectin polymeric mixtures (tCh-P, 3% w/v) was performed to attest the feasibility of the technique. Tubular structures with about 4.15 mm internal diameter and 1.55 mm wall thickness were produced. The structures are highly porous, presenting interconnected pores with average diameter of about 360 mu m. Seeding of human smooth muscle cells on the material was successfully achieved by using collagen gel to facilitate cell migration and retention inside the structure of the scaffold. The methodology herein proposed was successfully validated for the production of tubular constructs, opening new perspectives for the fabrication of matrices based on polymers that are passive of crosslinking with small molecules. Besides being an interesting approach to produce tubular scaffolds, this methodology can be considered an useful platform to obtain materials for drug screening and diagnostic studies. (c) 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 48510. (AU)

• Processo FAPESP: 17/01858-0 - Obtenção de substitutos vasculares por cultivo celular em suportes constituídos de quitosana complexada com alginato ou pectina: caracterização mecânica e cultura celular em matrizes tubulares
• Beneficiário: Fernanda Carla Bombaldi de Souza
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 13/26534-1 - Obtenção de enxertos vasculares por cultivo celular em suportes constituídos de quitosana complexada com outros polissacarídeos
• Beneficiário: Fernanda Carla Bombaldi de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado