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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

miR156-targeted SPL10 controls Arabidopsis root meristem activity and root-derived de novo shoot regeneration via cytokinin responses

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Autor(es):
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Barrera-Rojas, Carlos Hernan [1, 2] ; Braga Rocha, Gabriel Henrique [1] ; Polverari, Laura [3] ; Pinheiro Brito, Diego Armando [1] ; Batista, Diego Silva [4] ; Notini, Marcela M. [1] ; Ferreira da Cruz, Ana Claudia [4] ; Ortiz Morea, Edna Gicela [1, 2] ; Sabatini, Sabrina [3] ; Otoni, Wagner Campos [4] ; Silveira Nogueira, Fabio Tebaldi [1]
Número total de Autores: 11
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Biol Sci, Lab Mol Genet Plant Dev, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil
[2] State Univ Sao Paulo, Biosci Inst, BR-18618970 Botucatu, SP - Brazil
[3] Univ Roma La Sapienza, Lab Funct Genom & Prote Model Syst, Dipartimento Biol & Biotecnol, I-00185 Rome - Italy
[4] Univ Fed Vicosa, Dept Plant Biol, Plant Tissue Culture Lab BIOAGRO, BR-36570900 Vicosa, MG - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Journal of Experimental Botany; v. 71, n. 3, p. 934-950, JAN 23 2020.
Citações Web of Science: 0
Resumo

Root growth is modulated by different factors, including phytohormones, transcription factors, and microRNAs (miRNAs). MicroRNA156 and its targets, the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) genes, define an age-dependent pathway that controls several developmental processes, including lateral root emergence. However, it remains unclear whether miR156-regulated SPLs control root meristem activity and root-derived de novo shoot regeneration. Here, we show that MIR156 and SPL genes have opposing expression patterns during the progression of primary root (PR) growth in Arabidopsis, suggesting that age cues may modulate root development. Plants with high miR156 levels display reduced meristem size, resulting in shorter primary root (PRs). Conversely, plants with reduced miR156 levels show higher meristem activity. Importantly, loss of function of SPL10 decreases meristem activity, while SPL10 de-repression increases it. Meristem activity is regulated by SPL10 probably through the reduction of cytokinin responses, via the modulation of type-B ARABIDOPSIS RESPONSE REGULATOR1(ARR1) expression. We also show that SPL10 de-repression in the PRs abolishes de novo shoot regenerative capacity by attenuating cytokinin responses. Our results reveal a cooperative regulation of root meristem activity and root-derived de novo shoot regeneration by integrating age cues with cytokinin responses via miR156-targeted SPL10. (AU)

Processo FAPESP: 15/17892-7 - Estudo funcional de vias reguladas pelos microRNAs miR159 e miR319 durante o desenvolvimento de flores e frutos de tomateiro
Beneficiário:Fabio Tebaldi Silveira Nogueira
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/17441-3 - Controle molecular da arquitetura vegetal: interconexão entre microRNAs, fatores de transcrição e fitohormônios
Beneficiário:Fabio Tebaldi Silveira Nogueira
Modalidade de apoio: Auxílio à Pesquisa - Temático