Histopatology and HSP70 analysis of the midgut of Rhinocricus padbergi (Diplopoda) in the evaluation of the toxicity of two new metallic-insecticides

Autor(es)

de Souza, Raphael B. ^{[1, 2]} ; Moreira-de-Sousa, Cristina ^[2] ; Ansoar-Rodriguez, Yadira ^[2] ; Mancini Coelho, Maria Paula ^[2] ; de Souza, Cleiton Pereira ^[2] ; Bueno, Odair Correa ^[2] ; Fontanetti, Carmem S. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Civil Engn Architecture & Urban Design, Campinas, SP - Brazil

^[2] Sao Paulo State Univ, Inst Biosci, UNESP, Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Environmental Science and Pollution Research; v. 27, n. 3, p. 3023-3033, JAN 2020.
• Citações Web of Science: 2

Resumo

Millipedes are organisms of the edaphic fauna and have been used as bioindicators for the evaluation of pollutants in the environment, as they are in constant contact with the soil. This study used the millipede Rhinocricus padbergi as surrogate to evaluate the toxicity of two metallic-insecticides that has been developed for leaf-cutting ants management. Millipedes were exposed in terrariums containing different concentrations of the metallic-insecticides and, after periods of 21 and 90 days, three individuals from each terrarium were dissected in order to remove the midgut, the organ where absorption of nutrients and, consequently, toxic substances occurs. The toxic action of the metallic-insecticides was analyzed through qualitative and semi-quantitative analysis of morphophysiological alterations and by quantitative analysis of the HSP70 stress protein. The results showed that the metallic-insecticides may increase HSP70 labeling, although not at all concentrations and periods of exposure. Histopathological alterations were not significant at any concentration, indicating that the cytoprotective action of HSP70 is able to prevent severe damage to the midgut. It is therefore suggested that the metallic-insecticides are not toxic to the species studied here as no toxicity was observed under the conditions tested. In addition, stress protein localization in midgut helps understand how morphophysiological processes can potentially be affected by pesticide exposure. (AU)

• Processo FAPESP: 12/12019-5 - Avaliação do potencial tóxico de dois metalo-inseticidas em organismos não-alvos
• Beneficiário: Raphael Bastão de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado