X-ray fluorescence spectroscopy (XRF) applied to plant science: challenges towards in vivo analysis of plants

Autor(es)

Montanha, Gabriel Sgarbiero ^[1] ; Rodrigues, Eduardo Santos ^[1] ; Marques, Joao Paulo Rodrigues ^[1] ; de Almeida, Eduardo ^[1] ; dos Reis, Andre Rodrigues ^[2] ; Pereira de Carvalho, Hudson Wallace ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ctr Nucl Energy Agr CENA, Ave Centenario 303, BR-13416000 Piracicaba, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Rua Domingos Costa Lopes 780, BR-17602496 Tupa, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: METALLOMICS; v. 12, n. 2, p. 183-192, FEB 1 2020.
• Citações Web of Science: 0

Resumo

X-ray fluorescence spectroscopy (XRF) is an analytical tool used to determine the elemental composition in a myriad of sample matrices. Due to the XRF non-destructive feature, this technique may allow time-resolved plant tissue analyses under in vivo conditions, and additionally, the combination with other non-destructive techniques. In this study, we employed handheld and benchtop XRF to evaluate the elemental distribution changes in living plant tissues exposed to X-rays, as well as real-time uptake kinetics of Zn-(aq) and Mn-(aq) in soybean (Glycine max (L.) Merrill) stem and leaves, for 48 hours, combined with transpiration rate assessment on leaves by an infrared gas analyzer (IRGA). We found higher Zn content than Mn in stems. The latter micronutrient, in turn, presented higher concentration in leaf veins. Besides, both micronutrients were more concentrated in the first trifolium (i.e., youngest leaf) of soybean plants. Moreover, the transpiration rate was more influenced by circadian cycles than Zn and Mn uptake. Thus, XRF represents a convenient tool for in vivo nutritional studies in plants, and it can be coupled successfully to other analytical techniques. (AU)

• Processo FAPESP: 15/05942-0 - Investigação do metabolismo de nanomateriais absorvidos por plantas empregando-se espectroscopias vibracional e de Raios X
• Beneficiário: Hudson Wallace Pereira de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/16375-4 - Efeitos da aplicação foliar de La e Ce em plantas de soja
• Beneficiário: Eduardo Santos
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/19121-8 - EMU concedido no processo 2015/05942-0: sistema de microfluorescência de Raios X
• Beneficiário: Hudson Wallace Pereira de Carvalho
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários