Effect of photobiomodulation associated with cell therapy in the process of cutaneous regeneration in third degree burns in rats

Autor(es)

de Andrade, Ana Laura Martins ^[1] ; Brassolatti, Patricia ^[2] ; Luna, Genoveva Flores ^[3] ; Parisi, Julia Risso ^[1] ; Leal, Angela Merice de Oliveira ^[3] ; Frade, Marco Andrey Cipriani ^[4] ; Parizotto, Nivaldo Antonio ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Carlos UFSCar, Dept Physiotherapy, Sao Carlos - Brazil

^[2] Fed Univ Sao Carlos UFSCar, Dept Morphol & Pathol, Sao Carlos - Brazil

^[3] Fed Univ Sao Carlos UFSCar, Dept Med, Postgrad Program Biotechnol, Sao Carlos - Brazil

^[4] Univ Sao Paulo, Div Dermatol, Dept Internal Med, Ribeirao Preto Med Sch, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE; v. 14, n. 5 APR 2020.
• Citações Web of Science: 0

Resumo

Due to the complexity involved in the healing process of full thickness burns, the literature looks for alternatives to optimize tissue reconstruction. The objective of this study was to explore the action of photobiomodulation therapy associated with MSCs in the healing process of third degree burns. A total of 96 male Wistar rats were used, distributed in four groups with 24 animals each: Control Group, Laser Group, Cell Therapy Group, and Laser Group and Cell Therapy. The burn was performed with aluminum plate (150 degrees C). We performed analysis of wound contraction, histology, immunohistochemistry, birefringence analysis, and immunoenzymatic assay to evaluate tissue quality. Our results demonstrate that the association of the techniques is able to accelerate the repair process, modulating the inflammatory process, presenting a cutaneous tissue with better quality. Thus, we conclude that the use of photobiomodulation therapy associated with cell therapy is a promising treatment in the repair of total thickness burns. (AU)

• Processo FAPESP: 15/17648-9 - EFEITOS DA TERAPIA LASER DE BAIXA INTENSIDADE (660nm) ASSOCIADA À CÉLULAS TRONCO MESENQUIMAIS EM QUEIMADURAS DE TERCEIRO GRAU: ESTUDO "IN VITRO" E "IN VIVO".
• Beneficiário: Ana Laura Martins de Andrade
• Modalidade de apoio: Bolsas no Brasil - Doutorado