A novel Trichoderma reesei mutant RP698 with enhanced cellulase production

Autor(es)

Rodrigues Silva, Jean Carlos ^{[1, 2]} ; Santos Salgado, Jose Carlos ^[3] ; Vici, Ana Claudia ^[4] ; Ward, Richard John ^[3] ; Teixeira Moraes Polizeli, Maria Lourdes ^[4] ; Souza Guimaraes, Luis Henrique ^[4] ; Melo Furriel, Rosa Prazeres ^[3] ; Jorge, Joao Atilio ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Av Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Inst Fed Educ Ciencia & Tecnol Sao Paulo, Campus Sertazinho, Rua Amer Ambrosio 269, BR-14169263 Sertazinho, SP - Brazil

^[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Av Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Av Bandeirantes 3900, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 51, n. 2, p. 537-545, JUN 2020.
• Citações Web of Science: 0

Resumo

A new strain of Trichoderma reesei (teleomorph Hypocrea jecorina) with high cellulase production was obtained by exposing the spores from T. reesei QM9414 to an ultraviolet light followed by selecting fast-growing colonies on plates containing CMC (1% w/v) as the carbon source. The mutant T. reesei RP698 reduced cultivation period to 5 days and increased tolerance to the end-products of enzymatic cellulose digestion. Under submerged fermentation conditions, FPase, CMCase, and Avicelase production increased up to 2-fold as compared to the original QM9414 strain. The highest levels of cellulase activity were obtained at 27 degrees C after 72 h with Avicel (R), cellobiose, and sugarcane bagasse as carbon sources. The temperature and pH activity optima of the FPase, CMCase, and Avicelase were approximately 60 degrees C and 5.0, respectively. The cellulase activity was unaffected by the addition of 140 mM glucose in the enzyme assay. When T. reesei RP698 crude extract was supplemented by the addition of beta -glucosidase from Scytalidium thermophilum, a 2.3-fold increase in glucose release was observed, confirming the low inhibition by the end-product of cellulose hydrolysis. These features indicate the utility of this mutant strain in the production of enzymatic cocktails for biomass degradation. (AU)

• Processo FAPESP: 14/14415-0 - Enzimas lignocelulolíticas de fungos filamentosos: identificação, purificação, caracterização, relação estrutura-função e potencial de aplicação biotecnológica
• Beneficiário: Rosa dos Prazeres Melo Furriel
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/58482-2 - Estudo das propriedades bioquimicas das endoglucanases produzidas pelo fungo scytalidium thermophilum e seu potencial de aplicacao em complexos enzimaticos para hidrolise de biomassa
• Beneficiário: Jean Carlos Rodrigues da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 08/53249-8 - Enzimas de fungos termofílicos
• Beneficiário: João Atílio Jorge
• Modalidade de apoio: Auxílio à Pesquisa - Regular