Chitosan-based glycerol-plasticized membranes: bactericidal and fibroblast cellular growth properties

Autor(es):Mostrar menos -

Caroni, Joiciara Garcia ^[1] ; de Almeida Mattos, Alexia Victoria ^[2] ; Fernandes, Kelly Rossetti ^[3] ; Balogh, Debora Terezia ^[4] ; Muniz Renno, Ana Claudia ^[5] ; Okura, Monica Hitomi ^[2] ; Granato Malpass, Ana Claudia ^[2] ; Ferraresi, Cleber ^[1] ; Garcia, Livia Assis ^[1] ; Sanfelice, Rafaela Cristina ^[2] ; Pavinatto, Adriana ^[1]

Afiliação do(s) autor(es)

^[1] Brazil Univ, Sci & Technol Inst, 235 Carolina Fonseca St, BR-08230030 Sao Paulo, SP - Brazil

^[2] Fed Univ Triangulo Mineiro UFTM, Inst Technol & Exact Sci ICTE, Dept Chem Engn, 1250 Randolfo Borges Jr Ave, BR-38064200 Uberaba, MG - Brazil

^[3] Fed Univ Sao Carlos UFSCar, Dept Physiotherapy, Washington Luiz Rd, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Sao Paulo, Sao Carlos Inst Phys, 400 Trabalhador Sao Carlense Ave, BR-13566590 Sao Carlos, SP - Brazil

^[5] Fed Univ Sao Paulo UNIFESP, Dept Biosci, 136 Silva Jardim St, BR-11015020 Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: POLYMER BULLETIN; v. 78, n. 8 AUG 2020.
• Citações Web of Science: 0

Resumo

Chitosan-based glycerol-plasticized membranes were prepared aiming to develop low-cost and biocompatible material for wound dressing. They were produced by casting/solvent evaporation, and their formation mechanisms were attributed to intra- and inter-hydrogen bonds between materials. The results show that the glycerol acted as plasticizer and changed the proliferation of fibroblast cells on chitosan membranes. A high increase in the contact angle between water and membrane was observed comparing pure chitosan (more hydrophilic) and chitosan-glycerol (more hydrophobic) membranes. The angle values were found to be similar to 40 and similar to 130 degrees for chitosan and chitosan/glycerol 30% (w/w) membranes, respectively. In comparison with pure chitosan, glycerol-mixed membranes showed lower glass transition temperatures and are less brittle when handling. The antibacterial properties of such membranes against Gram-negative (E. coli) and Gram-positive (S. aureus and B. Cereus) microorganisms were tested, showing efficient growth inhibition. In vitro studies over fibroblast cells using plasticized membranes demonstrated good viability and enhanced cell proliferation in comparison with those composed of pure chitosan. Such results suggest that the as-prepared chitosan-glycerol membrane is a promising material for use as wound dressing in tissue engineering applications. (AU)

• Processo FAPESP: 17/19470-8 - Desenvolvimento de biossensor portátil para detecção não invasiva de ácido úrico como indicativo de hiperuricemia
• Beneficiário: Adriana Pavinatto da Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular