Keratin expression in gingival tissue and primary cultured gingival keratinocytes: Are there differences?

Autor(es)

Perillo Rosin, Flavia Cristina ^[1] ; Gonsalves, Heloisa ^[1] ; Santos, Anaeliza Figueiredo ^[1] ; Novaes, Cristina de Paula ^[1] ; Huang, Isaac ^[1] ; Zindel Deboni, Maria Cristina ^[2] ; Correa, Luciana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Pathol Dept, Av Prof Lineu Presses 2227, Cidade Univ, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dent Surg Dept, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 117, SEP 2020.
• Citações Web of Science: 0

Resumo

Objective: To investigate whether the process of primary gingival keratinocytes culture obtained from normal human gingiva modifies the expression of keratins (K) 10, K14, and K19. Design: Human gingival fragments were collected from healthy individuals in the same oral site. One part of the samples underwent an immunohistochemistry assay for K10, K14, and K19. The labeling in the epithelium was quantified using a semiautomated method. Another part was used for primary gingival keratinocytes isolation and growth in two-dimensional culture. These cells were also stained for K10, K14, and K19 using immunofluorescence and immunocytochemistry. Positive cells were counted, and the nuclei and cytoplasmatic labeling areas were quantified. Results: In the gingival tissue, a higher expression was found for K14 versus K10 (p < 0.001); K19 was negative in all samples. In gingival keratinocytes culture, K14 (89.6 %) had the highest expression with significant differences in relation to K10 (76.9 %, p < 0.01) and K19 (9.9 %, p < 0.01). The cells positive for K14 exhibited larger nuclei in comparison with K10 (p < 0.05) and K19 (p < 0.05), suggesting a more undifferentiated phenotype. K19 cells showed the largest cytoplasmatic labeling in relation to K10- (p < 0.05) and K14-positive (p < 0.05) cells. Conclusion: The process of growth in culture of gingival keratinocytes maintained the expression pattern of K10 and K14 observed in gingival tissues. However, this method induces the expression of K19, suggesting a potential transformation of the keratin network presented in the gingival keratinocytes during the formation of a monolayer in vitro. This reflects the dynamics of cell differentiation. (AU)

• Processo FAPESP: 18/19028-6 - Efeito da laserterapia de baixa potência sobre o estresse oxidativo e o reparo no DNA em células da mucosa oral expostas a agentes quimioterápicos e a radiação gama: estudo in vitro
• Beneficiário: Luciana Corrêa
• Modalidade de apoio: Auxílio à Pesquisa - Regular