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Dionizio, Aline
[1]
;
Melo, Carina Guimaraes Souza
[1]
;
Sabino-Arias, Isabela Tomazini
[1]
;
Araujo, Tamara Teodoro
[1]
;
Ventura, Talita Mendes Oliveira
[1]
;
Leite, Aline Lima
[1]
;
Souza, Sara Raquel Garcia
[2]
;
Santos, Erika Xavier
[2]
;
Heubel, Alessandro Domingues
[1]
;
Souza, Juliana Gadelha
[1]
;
Perles, Juliana Vanessa Colombo Martins
[2]
;
Zanoni, Jacqueline Nelisis
[2]
;
Buzalaf, Marilia Afonso Rabelo
[1]
Número total de Autores: 13
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil
[2] Univ Estadual Maringa, Dept Morphophysiol Sci, Maringa, Parana - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Science of The Total Environment; v. 741, NOV 1 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Fluoride (F) is largely employed in dentistry, in therapeutic doses, to control caries. However, excessive intake may lead to adverse effects in the body. Since F is absorbed mostly from the gastrointestinal tract (GIT), gastro-intestinal symptoms are the first signs following acute F exposure. Nevertheless, little is known about the mech-anistic events that lead to these symptoms. Therefore, the present study evaluated changes in the proteomic profile as well as morphological changes in the jejunum and ileum of rats upon acute exposure to F. Male rats re-ceived, by gastric gavage, a single dose of F containing 0 (control) or 25 mg/Kg for 30 days. Upon exposure to F, there was a decrease in the thickness of the tunic muscularis for both segments and a decrease in the thickness of the wall only for the ileum. In addition, a decrease in the density of HuC/D-IR neurons and nNOS-IR neurons was found for the jejunum, but for the ileum only nNOS-IR neurons were decreased upon F exposure. Moreover, SP-IR varicosities were increased in both segments, while VIP-IR varicosities were increased in the jejunum and de-creased in the ileum. As for the proteomic analysis, the proteins with altered expression were mostly negatively regulated and associated mainly with protein synthesis and energy metabolism. Proteomics also revealed alter-ations in proteins involved in oxidative/antioxidant defense, apoptosis and as well as in cytoskeletal proteins. Our results, when analyzed together, suggest that the gastrointestinal symptoms found in cases of acute F exposure might be related to the morphological alterations in the gut (decrease in the thickness of the tunica muscularis) that, at the molecular level, can be explained by alterations in the gut vipergic innervation and in proteins that regulate the cytoskeleton. (C) 2020 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 11/10233-7 - Avaliação da inervação entérica e análise proteômica do intestino delgado de ratos expostos a dose aguda ou crônica de fluoreto |
| Beneficiário: | Carina Guimarães de Souza Melo |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 16/09100-6 - Análise proteômica do jejuno e íleo em ratos expostos a dose aguda ou crônica de fluoreto. |
| Beneficiário: | Aline Dionizio Valle |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 12/16840-5 - Toxicologia do fluoreto: estudos proteômicos em intestino e fígado |
| Beneficiário: | Marília Afonso Rabelo Buzalaf |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |