Theranostic verteporfin- loaded lipid-polymer liposome for photodynamic applications

Autor(es):Mostrar menos -

Salles de Oliveira, Daphne Christine ^[1] ; de Freitas, Camila Fabiano ^[1] ; Calori, Italo Rodrigo ^[2] ; Goncalves, Renato Sonchini ^[1] ; Errerias Fernandes Cardinali, Camila Aparecida ^[3] ; Malacarne, Luis Carlos ^[4] ; Ravanelli, Maria Ida ^[5] ; de Oliveira, Hueder Paulo Moises ^[5] ; Tedesco, Antonio Claudio ^[2] ; Caetano, Wilker ^[1] ; Hioka, Noboru ^[1] ; Tessaro, Andre Luiz ^[6]

Afiliação do(s) autor(es)

^[1] Univ Estadual Maringa, Dept Chem, Res Nucleus Photodynam Therapy, BR-87020900 Maringa, Parana - Brazil

^[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Photobiol & Photomed Res Grp, Dept Chem, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Estadual Maringa, Dept Physiol Sci, BR-87020900 Maringa, Parana - Brazil

^[4] Univ Estadual Maringa, Dept Phys, BR-87020900 Maringa, Parana - Brazil

^[5] Fed Univ ABC, Ctr Nat & Human Sci, LACOMB, BR-09210580 Santo Andre, SP - Brazil

^[6] Fed Technol Univ Parana, Nucleus Ind Innovat, BR-86812460 Apucarana, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 212, NOV 2020.
• Citações Web of Science: 0

Resumo

In this study we report a novel theranostic lipid-polymer liposome, obtained from DPPC and the triblock copolymer F127 covalently modified with 5(6)-carboxyfluorescein (CF) for photodynamic applications. Due to the presence of F127, small unilamellar vesicle (SUV) liposomes were synthesized by a simple and fast thin-film hydration method without the need for an extrusion process. The vesicles have around 100 nm, low polydispersity and superb solution stability. The clinically used photosensitizer verteporfin (VP) was entrapped into the vesicles, mostly in monomeric form, with 90% loading efficiency. Stern-Volmer and fluorescence lifetime assays showed heterogeneous distribution of the VP and CF into the vesicles, ensuring the integrity of their individual photophysical properties. The theranostic properties were entirely photoactivatable and can be trigged by a unique wavelength (470 nm). The feasibility of the system was tested against the Glioblastoma multiforme cell line T98G. Cellular uptake by time-resolved fluorescence microscopy showed monomerized VP (monoexponential decay, 6.0 ns) at nucleus level, while CF was detected at the membrane by fluorescence microscopy. The strategy's success was supported by the reduction of 98% in the viability of T98G cells by the photoactivated lipid-polymer liposome with {[}VP] = 1.0 mu mol L-1 Therefore, the novel theranostic liposome is a potential system for use in cancer and ocular disease therapies. (AU)

• Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático