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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Identification and Characterization of New Bacteriophages to Control Multidrug-ResistantPseudomonas aeruginosaBiofilm on Endotracheal Tubes

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Autor(es):
Oliveira, Viviane C. [1, 2] ; Bim, Felipe L. [1] ; Monteiro, Rachel M. [1] ; Macedo, Ana Paula [2] ; Santos, Emerson S. [3] ; Silva-Lovato, Claudia H. [2] ; Paranhos, Helena F. O. [2] ; Melo, Luis D. R. [4] ; Santos, Silvio B. [4] ; Watanabe, Evandro [5, 1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Nursing Ribeirao Preto, Human Exposome & Infect Dis Network, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Dent Mat & Prostheses, Ribeirao Preto - Brazil
[3] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, Ribeirao Preto - Brazil
[4] Univ Minho, Ctr Biol Engn, Braga - Portugal
[5] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Restorat Dent, Ribeirao Preto - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN MICROBIOLOGY; v. 11, OCT 6 2020.
Citações Web of Science: 0
Resumo

Studies involving antimicrobial-coated endotracheal tubes are scarce, and new approaches to control multidrug-resistantPseudomonas aeruginosabiofilm on these devices should be investigated. In this study, five newP. aeruginosabacteriophages from domestic sewage were isolated. All of them belong to the orderCaudovirales,Myoviridaefamily. They are pH and heat stable and produce 27 to 46 particles after a latent period of 30 min at 37 degrees C. Their dsDNA genome (ranging from similar to 62 to similar to 65 kb) encodes 65 to 89 different putative proteins. They exhibit a broad lytic spectrum and infect 69.7% of theP. aeruginosastrains tested. All the bacteriophages were able to reduce the growth ofP. aeruginosastrains in planktonic form. The bacteriophages were also able to reduce the biofilm viability rates and the metabolic activity ofP. aeruginosastrains in a model of biofilms associated with endotracheal tubes. In addition, scanning electron microscopy micrographs showed disrupted biofilms and cell debris after treatment of bacteriophages, revealing remarkable biofilm reduction. The lytic activity on multidrug-resistantP. aeruginosabiofilm indicates that the isolated bacteriophages might be considered as good candidates for therapeutic studies and for the application of bacteriophage-encoded products. (AU)

Processo FAPESP: 19/13271-9 - Bacteriófagos com potencial aplicabilidade em tubos endotraqueais: atividades antibacteriana e antibiofilme
Beneficiário:Felipe Lazarini Bim
Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
Processo FAPESP: 18/09757-0 - Bacteriófagos com potencial aplicabilidade em tubos endotraqueais: atividades antibacteriana e antibiofilme
Beneficiário:Evandro Watanabe
Modalidade de apoio: Auxílio à Pesquisa - Regular