Porcine hemothropic mycoplasmas infection associated with productive impact in intensive pig production

Autor(es)

Petri, Fernando Antonio Moreira ^[1] ; Sonalio, Karina ^[1] ; de Souza Almeida, Henrique Meiroz ^[1] ; Ferraz, Maria Eugenia Silveira ^[2] ; Storino, Gabriel Yuri ^[1] ; de Souza, Mauro Rodrigo ^[3] ; Andre, Marcos Rogerio ^[1] ; de Oliveira, Luis Guilherme ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Agr & Veterinarian Sci, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP - Brazil

^[2] Evance Anim Hlth, Joao Baptista Queiroz Jr 447, BR-13098415 Campinas, SP - Brazil

^[3] Ceva Anim Hlth, Manoel Joaquim Filho 303, Paulinia, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PORCINE HEALTH MANAGEMENT; v. 6, n. 1 NOV 4 2020.
• Citações Web of Science: 0

Resumo

Background So far, three porcine hemoplasmas (PH) have been identified, namely Mycoplasma suis, Mycoplasma parvum, and Mycoplasma haemosuis. The first one is the main agent associated with porcine hemoplasmosis, a possible cause of economic losses in pig production. Thus, this work aimed to detect and quantify PH 16S rRNA in finishing pigs and to associate its load estimate with average daily weight gain (ADWG). For this purpose, whole blood samples from 318 pigs were collected at an age of 75 days (d0) when the pigs entered the finishing phase and 105 days later (d105). To calculate ADWG, the animals were weighed at the abovementioned dates. Then, DNA from blood samples were submitted to a qPCR targeting the 16S rRNA gene for PH. Spearman correlation test was performed to investigate potential associations between ADWG and the quantification values. Lastly, the molecular characterization of PH was done by sequencing the 23S rDNA gene. Results Out of the 318 samples, 190 (59.74%) were positive on d0, and 304 (95.6%) were positive on d105. A significant correlation was observed (p < 0.05), albeit with a low coefficient value (0.18), when comparing ADWG with quantification values on d105. The phylogenetic analysis based on the 23S rDNA gene showed that four sequences were closely related to M. parvum, and one sequence was positioned in the M. suis cluster. Conclusion Two PH, M. suis and M. parvum, were detected in a Brazilian pig farm. Moreover, increasing occurrence through time was observed, which may have affected the productive performance of positive animals, mainly at the end of the finishing phase, when antimicrobials are removed. (AU)

• Processo FAPESP: 19/14122-7 - Impacto produtivo associado a infecção por Mycoplasma suis em suínos de terminação no Brasil
• Beneficiário: Fernando Antônio Moreira Petri
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica