Biofilm Formation and Expression of Virulence Genes of Microorganisms Grown in Contact with a New Bioactive Glass

Autor(es)

Oliveira, Viviane de Cassia ^{[1, 2]} ; Souza, Marina Trevelin ^[3] ; Zanotto, Edgar Dutra ^[3] ; Watanabe, Evandro ^{[1, 4]} ; Coraca-Huber, Debora ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Nursing Ribeirao Preto, Human Exposome & Infect Dis Network HEID, Bandeirantes Ave 3900, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Dent Mat & Prostheses, Cafe Ave S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Mat Engn, Vitreous Mat Lab LaMaV, Rod Washington Luiz Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Restorat Dent, Cafe Ave S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[5] Med Univ Innsbruck, Dept Orthoped Surg, Expt Orthoped, Peter Mayr Str 4b, A-6020 Innsbruck - Austria

• Tipo de documento: Artigo Científico
• Fonte: PATHOGENS; v. 9, n. 11 NOV 2020.
• Citações Web of Science: 0

Resumo

Bioactive glass F18 (BGF18), a glass containing SiO2-Na2O-K2O-MgO-CaO-P2O5, is highly effective as an osseointegration buster agent when applied as a coating in titanium implants. Biocompatibility tests using this biomaterial exhibited positive results; however, its antimicrobial activity is still under investigation. In this study we evaluated biofilm formation and expression of virulence-factor-related genes in Candida albicans, Staphylococcus epidermidis, and Pseudomonas aeruginosa grown on surfaces of titanium and titanium coated with BGF18. C. albicans, S. epidermidis, and P. aeruginosa biofilms were grown on specimens for 8, 24, and 48 h. After each interval, the pH was measured and the colony-forming units were counted for the biofilm recovery rates. In parallel, quantitative real-time polymerase chain reactions were carried out to verify the expression of virulence-factor-related genes. Our results showed that pH changes of the culture in contact with the bioactive glass were merely observed. Reduction in biofilm formation was not observed at any of the studied time. However, changes in the expression level of genes related to virulence factors were observed after 8 and 48 h of culture in BGF18. BGF18 coating did not have a clear inhibitory effect on biofilm growth but promoted the modulation of virulence factors. (AU)

• Processo FAPESP: 18/17073-4 - 8th ASM Conference on Biofilms
• Beneficiário: Evandro Watanabe
• Modalidade de apoio: Auxílio à Pesquisa - Reunião - Exterior
• Processo FAPESP: 13/07793-6 - CEPIV - Centro de Ensino, Pesquisa e Inovação em Vidros
• Beneficiário: Edgar Dutra Zanotto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs