Interaction of Genetically Encoded Photosensitizers with Scintillating Nanoparticles for X-ray Activated Photodynamic Therapy

Autor(es)

Micheletto, Mariana C. ^[1] ; Guidelli, Eder J. ^[1] ; Costa-Filho, Antonio J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Fis, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 13, n. 2, p. 2289-2302, JAN 20 2021.
• Citações Web of Science: 2

Resumo

Photodynamic therapy (PDT) applications are limited by the low penetration of UV-visible light into biological tissues. Considering X-rays as an alternative to excite photosensitizers (PS) in a deeper tumor, an intermediate particle able to convert the X-ray energy into visible light (scintillating nanoparticle, ScNP) is necessary. Moreover, accumulation of PS in the target cells is also required. Genetically encoded proteins could be used as a photosensitizer, allowing the exclusive expression of PS inside the tumor cells. Here, the interaction of eGFP, KillerOrange, and KillerRed proteins with LaF3:Tb3+ ScNP was investigated, for the first time, in terms of its physicochemical and energy transfer properties. The protein structure, stability, and function were evaluated upon adverse physiological conditions and X-ray irradiation. Optimal parameters for energy transfer from ScNP to the proteins were investigated, paving the way for the use of genetically encoded photosensitizers for applications in X-ray activated photodynamic therapy. (AU)

• Processo FAPESP: 15/50366-7 - Resolving mechanistic details of peptide transport across membranes using crystallographic and non-crystallographic structural biology approaches
• Beneficiário: Antonio José da Costa Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/13016-6 - Ativação de Proteínas Fotossensíveis com Nanopartículas Radioluminescentes e Raios-X
• Beneficiário: Mariana Chaves Micheletto
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/20367-3 - Estudos estruturais e funcionais da proteína de organização e compactação do Golgi (GRASP) de Cryptococcus neoformans
• Beneficiário: Antonio José da Costa Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular