| Texto completo | |
| Autor(es): |
Ponce Ayala, Erika Toneth
[1]
;
Dias de Sousa, Fernanda Alves
[1]
;
Vollet-Filho, Jose Dirceu
[1]
;
Garcia, Marlon Rodrigues
[1]
;
de Boni, Leonardo
[1]
;
Bagnato, Vanderlei Salvador
[1]
;
Pratavieira, Sebastiao
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sao Carlos Inst Phys, Av Trab Sao Carlense, 400 Parque Arnold Schimidt, Sao Carlos, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ULTRASOUND IN MEDICINE AND BIOLOGY; v. 47, n. 4, p. 1032-1044, APR 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Sono-photodynamic therapy is a promising anticancer technique based on the combination of and photodynamic therapy to improve the cancer treatment effectiveness. This study was aimed at analyzing the effects of the sono-photodynamic (SPD) activity on protoporphyrin IX (PpIX) solution and PpIX-loaded rat liver. In vitro, PpIX 5 mu M solutions were irradiated with light (635 nm, 30-50 mW/cm(2)), ultrasound (1 MHz, 1-2 W/cm(2)) and both. The PpIX absorption spectra recorded over exposure time revealed that the PpIX decay rate induced by SPD activity (combined irradiation) was approximately the sum of those induced by photodynamic and sonodynamic activity. In vivo, rats were intraperitoneally injected with 5-aminolevulinic acid at the dose of 500 mg/kg weight. After 3 h of injection, the PpIX-loaded livers were irradiated with light (635 nm, 180 +/- 9 J/cm(2)), ultrasound (1.0 MHz, 770 +/- 40 J/cm(2)) and both using a single probe capable of illuminating and sonicating the liver simultaneously. After 30 h, the liver damage induced by each protocol was analyzed histologically. It was found that a greater necrosis depth was induced by the SPD activity. These results suggest that the SPD activity could improve the PpIX decay rate and have greater scope than photodynamic or sonodynamic activity. Further studies should be performed to gain a better understanding of this protocol. (E-mail: eriponce@usp.br) (C) 2020 World Federation for Ultrasound in Medicine \& Biology. All rights reserved. (AU) | |
| Processo FAPESP: | 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida |
| Beneficiário: | Vanderlei Salvador Bagnato |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica |
| Beneficiário: | Vanderlei Salvador Bagnato |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |