Community composition and functional prediction of prokaryotes associated with sympatric sponge species of southwestern Atlantic coast

Autor(es)

Hardoim, C. C. P. ^[1] ; Ramaglia, A. C. M. ^[1] ; Lobo-Hajdu, G. ^[2] ; Custodio, M. R. ^[3]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Inst Biosci, Coastal Campus Sao Vicente, Sao Paulo - Brazil

^[2] Univ Estado Rio De Janeiro, Biol Inst Roberto Alcantara Gomes, Dept Genet, Rio De Janeiro - Brazil

^[3] Univ Sao Paulo, Biosci Inst & NP Biomar, Dept Physiol, Ctr Marine Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 MAY 5 2021.
• Citações Web of Science: 1

Resumo

Prokaryotes contribute to the health of marine sponges. However, there is lack of data on the assembly rules of sponge-associated prokaryotic communities, especially for those inhabiting biodiversity hotspots, such as ecoregions between tropical and warm temperate southwestern Atlantic waters. The sympatric species Aplysina caissara, Axinella corrugata, and Dragmacidon reticulatum were collected along with environmental samples from the north coast of SAo Paulo (Brazil). Overall, 64 prokaryotic phyla were detected; 51 were associated with sponge species, and the dominant were Proteobacteria, Bacteria (unclassified), Cyanobacteria, Crenarchaeota, and Chloroflexi. Around 64% and 89% of the unclassified operational taxonomical units (OTUs) associated with Brazilian sponge species showed a sequence similarity below 97%, with sequences in the Silva and NCBI Type Strain databases, respectively, indicating the presence of a large number of unidentified taxa. The prokaryotic communities were species-specific, ranging 56%-80% of the OTUs and distinct from the environmental samples. Fifty-four lineages were responsible for the differences detected among the categories. Functional prediction demonstrated that Ap. caissara was enriched for energy metabolism and biosynthesis of secondary metabolites, whereas D. reticulatum was enhanced for metabolism of terpenoids and polyketides, as well as xenobiotics' biodegradation and metabolism. This survey revealed a high level of novelty associated with Brazilian sponge species and that distinct members responsible from the differences among Brazilian sponge species could be correlated to the predicted functions. (AU)

• Processo FAPESP: 16/17189-7 - Holobioma das esponjas marinhas, efeitos das mudanças climáticas e potenciais biotecnológicos: uma abordagem holística e multidisciplinar
• Beneficiário: Cristiane Cassiolato Pires Hardoim
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Jovens Pesquisadores
• Processo FAPESP: 17/10157-5 - Holobioma das esponjas marinhas, efeitos das mudanças climáticas e potenciais biotecnológicos: uma abordagem holística e multidisciplinar
• Beneficiário: Cristiane Cassiolato Pires Hardoim
• Modalidade de apoio: Bolsas no Brasil - Programa de Pesquisa sobre Mudanças Climáticas Globais - Jovens Pesquisadores
• Processo FAPESP: 18/12937-0 - Holobioma das esponjas marinhas, efeitos das mudanças climáticas e potenciais biotecnológicos: uma abordagem holística e multidisciplinar
• Beneficiário: Andressa Cristina Ramaglia da Mota
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 17/11380-0 - EMU concedido no processo 16/18740-9:SERVIDOR HPE DL 580 Gen9
• Beneficiário: Marcelo Urbano Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários