Levels of cardiorespiratory fitness in men exerts strong impact on lymphocyte function after mitogen stimulation

Autor(es)

Dorneles, Gilson P. ^[1] ; Lira, Fabio Santos ^[2] ; Romao, Pedro R. T. ^[1] ; Krueger, Karsten ^[3] ; Rosa-Neto, Jose Cesar ^[4] ; Peres, Alessandra ^[1] ; Antunes, Barbara Moura ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Ciencias Saude Porto Alegre, Cellular & Mol Immunol Lab, Porto Alegre, RS - Brazil

^[2] Univ Estadual Paulista UNESP, Dept Phys Educ, Exercise & Lmmunometab Res Grp, Postgrad Program Movement Sci, BR-19060900 Presidente Prudente, SP - Brazil

^[3] Justus Liebig Univ Giessen, Dept Exercise Physiol & Sports Therapy, Giessen - Germany

^[4] Univ Sao Paulo, Inst Biomed Sci, Immunometab Res Grp, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Physiology; v. 130, n. 4, p. 1133-1142, APR 2021.
• Citações Web of Science: 0

Resumo

Relationship between lymphocyte function and cardiorespiratory fitness (CRF) is well-documented at rest; however, upon mitogen stimulation the proliferation and cytokine production alters, but knowledge is incipient about lymphocyte responses after mitogen stimulus according to CRF. So, the purpose of the present study was to analyze the lymphocyte function according to the physical fitness status of healthy young men. The study is divided in two experiments being the first analyzing the lymphocyte phenotypes profile and the inflammatory responses, according to CRF, in lymphocyte cell cultures treated for 48 h with concanavalin A (ConA). The second experiment analyzed the proliferation, reactive oxygen species production, viability, and mitochondrial polarization state in lymphocytes treated with ConA in different concentrations, considering the CRF levels. The results showed a difference in the percentage of total lymphocytes expression between groups (P = 0.011) observing a lower lymphocytes T expression in the group with high maximal oxygen consumption (<V>over doto(2max)) when compared with the moderate <V>over doto(2max) group. When treated with ConA, the lymphocytes of the low <V>over doto(2max) group released higher TNF-alpha concentration (P = 0.032), reflecting an . elevated TNF-alpha/IL-10 ratio (P = 0.055), parallel with lower IL-6 production (P = 0.027), mainly when compared V with the moderate <V>over doto(2max) group. In addition, there is a positive relationship between <V>over doto(2max) and IL-6 production (r = 0.507; P = 0.016), whereas the percentage of total lymphocytes (LyT%) shows a negative trend with <V>over doto(2max) (r = -0.497; P = 0.060). Also, individuals with lower <V>over doto(2max) showed reduced absolute and relative ROS production, lower cell proliferation, and higher mitochondrial membrane depolarization. In conclusion, cardiorespiratory fitness degree exerts a strong impact on lymphocyte function after mitogen stimulation. NEW \& NOTEWORTHY The innovation of the research is to elucidate the impact of different physical fitness status on metabolism, cell proliferation, and lymphocyte activity and, consequently, on the specific inflammatory response against a mitogen. (AU)

• Processo FAPESP: 14/08003-1 - Envolvimento do NF-kB e PPAR-gamma na resposta inflamatória e metabólica em monócitos de indivíduos sedentários, fisicamente ativos e treinados
• Beneficiário: Barbara de Moura Mello Antunes
• Modalidade de apoio: Bolsas no Brasil - Doutorado