Enhanced aluminum tolerance in sugarcane: evaluation of SbMATE overexpression and genome-wide identification of ALMTs in Saccharum spp.

Autor(es):Mostrar menos -

Ribeiro, Ana Paula ^[1] ; Vinecky, Felipe ^[1] ; Duarte, Karoline Estefani ^{[2, 1]} ; Santiago, Thais Ribeiro ^{[1, 3]} ; das Chagas Noqueli Casari, Raphael Augusto ^[1] ; Hell, Aline Forgatti ^[2] ; Dias Brito da Cunha, Barbara Andrade ^[1] ; Martins, Polyana Kelly ^[1] ; Centeno, Danilo da Cruz ^[2] ; de Oliveira Molinari, Patricia Abrao ^[1] ; de Almeida Cancado, Geraldo Magela ^[4] ; de Magalhaes, Jurandir Vieira ^[5] ; Kobayashi, Adilson Kenji ^[1] ; de Souza, Wagner Rodrigo ^{[2, 1]} ; Correa Molinari, Hugo Bruno ^[1]

Afiliação do(s) autor(es)

^[1] Embrapa Agroenergy, Genet & Biotechnol Lab, BR-70770901 Brasilia, DF - Brazil

^[2] Fed Univ ABC, Ctr Nat Sci & Humanities, BR-09606045 Sao Bernardo Do Campo, SP - Brazil

^[3] Univ Brasilia, Phytopathol Dept, BR-70910900 Brasilia, DF - Brazil

^[4] AC Unicamp Univ City, Embrapa GenClima, Ctr Genet Engn & Mol Biol, BR-13083886 Campinas, SP - Brazil

^[5] Embrapa Maize & Sorghum, Appl Biol Ctr NBA, BR-35701970 Sete Lagoas, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC PLANT BIOLOGY; v. 21, n. 1 JUN 29 2021.
• Citações Web of Science: 0

Resumo

BackgroundA major limiting factor for plant growth is the aluminum (Al) toxicity in acidic soils, especially in tropical regions. The exclusion of Al from the root apex through root exudation of organic acids such as malate and citrate is one of the most ubiquitous tolerance mechanisms in the plant kingdom. Two families of anion channels that confer Al tolerance are well described in the literature, ALMT and MATE family.ResultsIn this study, sugarcane plants constitutively overexpressing the Sorghum bicolor MATE gene (SbMATE) showed improved tolerance to Al when compared to non-transgenic (NT) plants, characterized by sustained root growth and exclusion of aluminum from the root apex based on the result obtained with hematoxylin staining. In addition, genome-wide analysis of the recently released sugarcane genome identified 11 ALMT genes and molecular studies showed potential new targets for aluminum tolerance.ConclusionsOur results indicate that the transgenic plants overexpressing the Sorghum bicolor MATE has an improved tolerance to Al. The expression profile of ALMT genes revels potential candidate genes to be used has an alternative for agricultural expansion in Brazil and other areas with aluminum toxicity in poor and acid soils. (AU)

• Processo FAPESP: 19/16226-4 - Superexpressão de genes relacionados a síntese de poliois em Setaria
• Beneficiário: Karoline Estefani Duarte
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/15576-9 - Metabolismo do carbono em plantas sob diferentes perspectivas: buscando alternativas para aumento de produtividade
• Beneficiário: Danilo da Cruz Centeno
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
• Processo FAPESP: 19/04878-7 - Biotecnologia aplicada ao melhoramento genético de gramíneas para a produção de biocombustíveis
• Beneficiário: Wagner Rodrigo de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores