Genome-wide association mapping reveals race-specific SNP markers associated with anthracnose resistance in carioca common beans

Autor(es)

de Almeida, Caleo Panhoca ^[1] ; de Carvalho Paulino, Jean Fausto ^[1] ; Ferrari Barbosa, Caio Cesar ^[1] ; Cunha Goncalves, Gabriel de Moraes ^[2] ; Fritsche-Neto, Roberto ^[3] ; Morais Carbonell, Sergio Augusto ^[2] ; Chiorato, Alisson Fernando ^[2] ; Benchimol-Reis, Luciana Lasry ^[1]

Afiliação do(s) autor(es)

^[1] Inst Agron IAC, Ctr Pesquisa Recursos Genet Vegetais, Campinas, SP - Brazil

^[2] Inst Agron IAC, Ctr Graos & Fibras, Campinas, SP - Brazil

^[3] Univ Sao Paulo ESALQ USP, Dept Genet, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 16, n. 5 MAY 19 2021.
• Citações Web of Science: 0

Resumo

Brazil is the largest consumer of dry edible beans (Phaseolus vulgaris L.) in the world, 70% of consumption is of the carioca variety. Although the variety has high yield, it is susceptible to several diseases, among them, anthracnose (ANT) can lead to losses of up to 100% of production. The most effective strategy to overcome ANT, a disease caused by the fungus Colletotrichum lindemuthianum, is the development of resistant cultivars. For that reason, the selection of carioca genotypes resistant to multiple ANT races and the identification of loci/markers associated with genetic resistance are extremely important for the genetic breeding process. Using a carioca diversity panel (CDP) with 125 genotypes and genotyped by BeadChip BARCBean6K\_3 and a carioca segregating population AM (AND-277 x IAC-Milenio) genotyped by sequencing (GBS). Multiple interval mapping (MIM) and genome-wide association studies (GWAS) were used as mapping tools for the resistance genes to the major ANT physiological races present in the country. In general, 14 single nucleotide polymorphisms (SNPs) showed high significance for resistance by GWAS, and loci associated with multiple races were also identified, as the Co-3 locus. The SNPs ss715642306 and ss715649427 in linkage disequilibrium (LD) at the beginning of chromosome Pv04 were associated with all the races used, and 16 genes known to be related to plant immunity were identified in this region. Using the resistant cultivars and the markers associated with significant quantitative resistance loci (QRL), discriminant analysis of principal components (DAPC) was performed considering the allelic contribution to resistance. Through the DAPC clustering, cultivar sources with high potential for durable anthracnose resistance were recommended. The MIM confirmed the presence of the Co-1(4)locus in the AND-277 cultivar which revealed that it was the only one associated with resistance to ANT race 81. Three other loci were associated with race 81 on chromosomes Pv03, Pv10, and Pv11. This is the first study to identify new resistance loci in the AND-277 cultivar. Finally, the same Co-1(4)locus was also significant for the CDP at the end of Pv01. The new SNPs identified, especially those associated with more than one race, present great potential for use in marker-assisted and early selection of inbred lines. (AU)

• Processo FAPESP: 17/24711-4 - Estudo de associação ampla do genoma para resistência à Fusarium oxysporum f. sp. phaseoli e para cor de tegumento em feijoeiro comum
• Beneficiário: Luciana Lasry Benchimol-Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/19670-2 - Genômica populacional e GWAS para resistência à doenças e identificação de regiões de introgressão de pool andino em acessos de feijão carioca
• Beneficiário: Caléo Panhoca de Almeida
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto