Genome-wide association mapping reveals new loci associated with light-colored seed coat at harvest and slow darkening in carioca beans

Autor(es):Mostrar menos -

de Almeida, Caleo Panhoca ^[1] ; Santos, Isabella Laporte ^[1] ; de Carvalho Paulino, Jean Fausto ^[1] ; Ferrari Barbosa, Caio Cesar ^[1] ; Augusto Pereira, Cassia Cristina ^[1] ; Limonta Carvalho, Cassia Regina ^[1] ; Cunha Goncalves, Gabriel de Moraes ^[2] ; Song, Qijian ^[3] ; Morais Carbonell, Sergio Augusto ^[2] ; Chiorato, Alisson Fernando ^[2] ; Benchimol-Reis, Luciana Lasry ^[1]

Afiliação do(s) autor(es)

^[1] Agron Inst IAC, Nat Ctr Plant Genet, Common Bean Genet Grp, Campinas, SP - Brazil

^[2] Agron Inst IAC, Grain & Fiber Ctr, Common Bean Breeding Grp, Campinas, SP - Brazil

^[3] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD - USA

• Tipo de documento: Artigo Científico
• Fonte: BMC PLANT BIOLOGY; v. 21, n. 1 JUL 20 2021.
• Citações Web of Science: 0

Resumo

Background Common bean (Phaseolus vulgaris L.) is a legume whose grain can be stored for months, a common practice among Brazilian growers. Over time, seed coats become darker and harder to cook, traits that are undesirable to consumers, who associate darker-colored beans with greater age. Like commercial pinto and cranberry bean varieties, carioca beans that have darker seeds at harvest time and after storage are subject to decreased market values. Results The goal of our study was to identify the genetic control associated with lightness of seed coat color at harvest (HL) and with tolerance to post-harvest seed coat darkening (PHD) by a genome-wide association study. For that purpose, a carioca diversity panel previously validated for association mapping studies was used with 138 genotypes and 1,516 high-quality SNPs. The panel was evaluated in two environments using a colorimeter and the CIELAB scale. Shelf storage for 30 days had the most expressive results and the L{*} (luminosity) parameter led to the greatest discrimination of genotypes. Three QTL were identified for HL, two on chromosome Pv04 and one on Pv10. Regarding PHD, results showed that genetic control differs for L{*} after 30 days and for the Delta L{*} (final L{*}-initial L{*}); only Delta L{*} was able to properly express the PHD trait. Four phenotypic classes were proposed, and five QTL were identified through six significant SNPs. Conclusions Lightness of seed coat color at harvest showed an oligogenic inheritance corroborated by moderate broad-sense heritability and high genotypic correlation among the experiments. Only three QTL were significant for this trait - two were mapped on Pv04 and one on Pv10. Considering the Delta L, six QTL were mapped on four different chromosomes for PHD. The same HL QTL at the beginning of Pv10 was also associated with Delta L{*} and could be used as a tool in marker-assisted selection. Several candidate genes were identified and may be useful to accelerate the genetic breeding process. (AU)

• Processo FAPESP: 18/20992-1 - Estudo do controle genético do escurecimento tardio e de coloração do tegumento em grãos de feijão do tipo carioca
• Beneficiário: Cassia Cristina Augusto Pereira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/24711-4 - Estudo de associação ampla do genoma para resistência à Fusarium oxysporum f. sp. phaseoli e para cor de tegumento em feijoeiro comum
• Beneficiário: Luciana Lasry Benchimol-Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/19670-2 - Genômica populacional e GWAS para resistência à doenças e identificação de regiões de introgressão de pool andino em acessos de feijão carioca
• Beneficiário: Caléo Panhoca de Almeida
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto