Antiproliferative Effects of Pterodon pubescens Extract and Isolated Diterpenes in HaCaT Cells

Autor(es)

Basting, Rosanna Tarkany ^{[1, 2, 3, 4]} ; de Oliveira Sousa, Ilza Maria ^{[1, 2, 3]} ; Butterweck, Veronika ^[5] ; Foglio, Mary Ann ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Pharmaceut Sci FCF, UNICAMP, Cidade Univ Zeferino Vaz Rua Candido Portinari 20, BR-13083871 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Fac Med Sci FCM, Postgrad Program Med Sci, UNICAMP, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Chem Biol & Agr Res Ctr CPQBA, Paulinia, SP - Brazil

^[4] Sao Leopoldo Mand Inst & Res Ctr, Lab Neuroimmune Interface Pain Res, Campinas, SP - Brazil

^[5] Univ Appl Sci Northwestern Switzerland FHNW, Sch Life Sci, Inst Pharma Technol, Muttenz - Switzerland

• Tipo de documento: Artigo Científico
• Fonte: Planta Medica; v. 87, n. 03, p. 218-224, MAR 2021.
• Citações Web of Science: 0

Resumo

Pterodon pubescens fruits are popularly used because of their analgesic and anti-inflammatory actions, which are attributed to the isolated compounds with a vouacapan skeleton. This work aimed to evaluate the antiproliferative and anti-inflammatory effects of a P. pubescens fruit dichloromethane extract and the vouacapan diterpene furan isomers mixture (1:1) (6 alpha -hydroxy-7 beta -acetoxy-vouacapan-17 beta -oate methyl ester and 6 alpha -acetoxy-7 beta -hydroxy-vouacapan-17 beta -oate methyl ester isomers) in HaCaT cells using the cell migration and the BrDU incorporation assay. Levels of IL-8 were measured by ELISA after TNF- alpha stimulation. HPLC/DAD analysis of the extract revealed the expressive presence of vouacapan diterpene furan isomers mixture. P. pubescens extract (1.5625-25 mu g/mL) and vouacapan diterpene furan isomers mixture (3.125-50 mu M) inhibited cell proliferation as indicated by a decreased BrdU-incorporation. For the evaluation of cell migration, time-lapse microscopy was used. P. pubescens presented inhibition on cell migration at all concentrations tested (3.125-12.5 mu g/mL), whereas for the VDFI mixture, the inhibition was only observed at the highest concentrations (12.5 and 25 mu M) tested. Furthermore P. pubescens extract and vouacapan diterpene furan isomers mixture significantly decreased IL-8 levels. Our results showed antiproliferative and anti-inflammatory effects on HaCaT cells treated with the extract and the vouacapan isomers mixture, without affecting cell viability. These activities could be attributed to the voucapan molecular structures. In conclusion, topical products developed of P. pubescens extract or the voucapan isomers mixture should be further studied as a potential product for local treatment against hyperproliferative lesions as in psoriasis vulgaris, representing an alternative treatment approach. (AU)

• Processo FAPESP: 15/08600-2 - Pterodon pubescens Benth. e Cordia verbenacea DC: avaliação do efeito sinérgico na atividade anti-inflamatória e estudos de mecanismos de ação e sinalização celular
• Beneficiário: Rosanna Tarkany Basting
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 16/23816-4 - Modulação in vitro da expressão gênica de alvos inflamatórios pela associação de Pterodon pubescens Benth. e Cordia verbenacea DC
• Beneficiário: Rosanna Tarkany Basting
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto