admium-induced transgenerational effects on tomato plants: A gift from parents to progenie

Autor(es)

Nogueira, Marina Lima ^[1] ; Carvalho, Marcia Eugenia Amaral ^[1] ; Ferreira, Joao Marcos Martins ^[2] ; Bressanin, Leticia Aparecida ^[2] ; Piotto, Katherine Derlene Batagin ^[1] ; Piotto, Fernando Angelo ^[3] ; Marques, Deyvid Novaes ^[1] ; Barbosa, Sandro ^[2] ; Azevedo, Ricardo Antunes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo ESALQ USP, Dept Genet, Escola Super Agr Luiz de Queiroz, Ave Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Fed Alfenas UNIFAL, Inst Ciencias Nat, Rua Gabriel Monteiro 700, BR-37130000 Alfenas, MG - Brazil

^[3] Univ Sao Paulo ESALQ USP, Dept Prod Vegetal, Escola Super Agr Luiz de Queiroz, Ave Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Science of The Total Environment; v. 789, OCT 1 2021.
• Citações Web of Science: 5

Resumo

The present study aimed to investigate the Cd-induced transgenerational effects on plants. G rafted tomato plants, which exhibited the same cultivar as scion and distinct cultivars with contrasting Cd-tolerance as rootstocks, were grown in soil without and with artificial addition of Cd (less than 2.0, and 6.9 mg kg(-1) of Cd, respectively) in a pot experiment carried out in a greenhouse. Their fruits were harvested to extract seeds (i.e., the progenies), which were sown over either Cd-free (control) or Cd-containing germitest paper (germination testing paper with 0 and 35 mu M of CdCl2, respectively) and grown in a growth chamber. The immediate progeny oral' grafting combinations from stressed plants presented an elevated germinability, despite high internal Cd concentration. When sown in Cd-containing germitest paper, the immediate progeny of plants grown in soil with no Cd addition was generally able to maintain or even increase the content of carotenoids and chlorophylls a and b (up to 93.3, 62.8 and 76.1%, respectively), indicating a Cd-induced hormetic effect on photosynthetic pigments. Two of the grafting combinations from stressed plants yielded seeds that generated seedlings with enhanced dry mass when they were sown in Cd-free media (similar to 41%), suggesting a Cd-induced transgenerational enhancement of biomass production. Because only one tomato cultivar was used as scion, data indicated that type and degree of Cdinduced transgenerational effects depend strongly on signals generated and/or processed in roots of the parental plants. When sown in Cd-contaminated germitest paper, the immediate progeny of Cd-treated plants presented major reductions in the leaf area (35-69%) and content of photosynthetic pigments (57-93%) in comparison to the progeny from control plants. However, one of the grafting combinations exhibited satisfactory performance after ``double' exposure to Cd, showing 91% of the biomass that was produced in the seedlings of control seeds from control plants. Further investigation indicated that adjustments in the chlorophyll fluorescence behavior might counterbalance losses in leaf pigments and area. Taken together, our data provide new insights on the origin, outcomes and mode of action of the Cd-induced transgenerational effects. (C) 2021 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 16/05788-3 - Caracterização de mecanismos de tolerância ao Cd em raízes de tomateiros usando porta-enxertos tolerantes
• Beneficiário: Katherine Derlene Batagin Piotto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/20706-9 - Identificação de proteínas-chave à tolerância ao cádmio: análise proteômica de cultivares de tomateiro sensível e tolerante ao Cd, e plantas enxertadas reciprocamente
• Beneficiário: Deyvid Novaes Marques
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 20/00133-4 - Alteração transgeracional causada pelo cádmio em plantas: um presente de pais para filhos
• Beneficiário: Marcia Eugenia Amaral de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/05544-0 - Estudo da cinética de absorção e do acúmulo de cádmio em cultivares de tomateiro
• Beneficiário: Deyvid Novaes Marques
• Modalidade de apoio: Bolsas no Brasil - Doutorado