Proteomic characterization of Naja mandalayensis venom

Autor(es)

Beraldo Neto, Emidio ^{[1, 2]} ; Coelho, Guilherme Rabelo ^[1] ; Sciani, Juliana Mozer ^[3] ; Pimenta, Daniel Carvalho ^[1]

Afiliação do(s) autor(es)

^[1] Butantan Inst, Lab Biochem & Biophys, Sao Paulo, SP - Brazil

^[2] Butantan Inst, Grad Program Sci Toxinol, Sao Paulo, SP - Brazil

^[3] Sao Francisco Univ, Multidisciplinary Res Lab, Braganca Paulista, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 27, JUL 30 2021.
• Citações Web of Science: 0

Resumo

Abstract Background Naja mandalayensis is a spitting cobra from Myanmar. To the best of our knowledge, no studies on this venom composition have been conducted so far. On the other hand, few envenomation descriptions state that it elicits mainly local inflammation in the victims’ eyes, the preferred target of this spiting cobra. Symptoms would typically include burning and painful sensation, conjunctivitis, edema and temporary loss of vision. Methods We have performed a liquid-chromatography (C18-RP-HPLC) mass spectrometry (ESI-IT-TOF/MS) based approach in order to biochemically characterize N. mandalayensis venom. Results A wide variety of three-finger toxins (cardiotoxins) and metallopeptidases were detected. Less abundant, but still representative, were cysteine-rich secretory proteins, L-amino-acid oxidases, phospholipases A2, venom 5'-nucleotidase and a serine peptidase inhibitor. Other proteins were present, but were detected in a relatively small concentration. Conclusion The present study set the basis for a better comprehension of the envenomation from a molecular perspective and, by increasing the interest and information available for this species, allows future venom comparisons among cobras and their diverse venom proteins. (AU)

• Processo FAPESP: 15/13142-3 - Bioprospecção do muco e do veneno da raia marinha Dasyatis americana
• Beneficiário: Guilherme Rabelo Coelho
• Modalidade de apoio: Bolsas no Brasil - Doutorado