RNA-Seq analysis of the blue light-emitting Orfelia fultoni (Diptera: Keroplatidae) suggest photoecological adaptations at the molecular level

Autor(es)

Amaral, Danilo T. ^[1] ; Johnson, Carl H. ^[2] ; Viviani, Vadim R. ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Grad Sch Biotechnol & Environm Monitoring UFSCar, Sorocaba, SP - Brazil

^[2] Vanderbilt Univ, Dept Biol Sci, Nashville, TN 37235 - USA

^[3] Fed Univ Sao Carlos UFSCar, Grad Sch Evolut Genet & Mol Biol, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Comparative Biochemistry and Physiology D-Genomics & Proteomics; v. 39, SEP 2021.
• Citações Web of Science: 0

Resumo

Bioluminescence in Diptera is found in the Keroplatidae family, within Arachnocampininae and Keroplatinae subfamilies, with reported occurrences in Oceania, Eurasia, and Americas. Larvae of Orfelia fultoni, which inhabit stream banks in the Appalachian Mountains, emit the bluest bioluminescence among insects, using it for prey attraction, similarly to Arachnocampa spp. Although bioluminescence has a similar prey attraction function, the systems of Arachonocampininae and Keroplatinae subfamilies are morphologically/biochemically distinct, indicating different evolutionary origins. To identify the possible coding genes associated with physiological control, ecological adaptations, and origin/evolution of bioluminescence in the Keroplatinae subfamily, we performed the RNA-Seq analysis of O. fultoni larvae during day and night and compared it with the tran-scriptomes of Arachnocampa luminosa, and reanalyzed the previously published proteomic data of O. fultoni against the RNA-Seq dataset. The abundance of chaperones/heat-shock and hexamerin gene products at night and in luciferase enriched fractions supports their possible association and participation in bioluminescence. The low diversity of copies/families of opsins indicate a simpler visual system in O. fultoni. Noteworthy, gene products associated with silk protein biosynthesis in Orfelia were more similar to Lepidoptera than to the Arachnocampa, indicating that, similarly to the bioluminescent systems, at some point, the biochemical apparatus for web construction may have evolved independently in Orfelia and Arachnocampa. (AU)

• Processo FAPESP: 14/20176-9 - Transcriptoma das lanternas de larva e adulto e do corpo gorduroso de larva de Elateroidea (Coleoptera)
• Beneficiário: Danilo Trabuco do Amaral
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/50583-5 - Identification of the luciferases and acessory proteins of the bioluminescent system of Orfelia fultonii (Diptera : Keroplatidae): transcriptional and proteomic analysis
• Beneficiário: Vadim Viviani
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/05426-8 - Bioluminescência de artrópodes: diversidade biológica em biomas brasileiros; origem bioquímica; evolução estrutural/funcional de luciferases; diferenciação molecular das lanternas; aplicações biotecnológicas, ambientais e educacionais
• Beneficiário: Vadim Viviani
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/25051-2 - Perfil transcricional de Orfelia fultoni (Diptera: Keroplatidae): investigando genes e rotas envolvidas com a bioluminescência em Diptera
• Beneficiário: Danilo Trabuco do Amaral
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado