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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

nvestigation of Protein Biomarkers and Oxidative Stress in Pinirampus pirinampu Exposed to Mercury Species from the Madeira River, Amazon-Brazi

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Autor(es):
Souza Vieira, Jose Cavalcante [1] ; de Oliveira, Grasieli [1] ; Cavallini, Nubya Goncalves [1] ; Braga, Camila Pereira [2] ; Adamec, Jiri [2] ; Zara, Luiz Fabricio [3] ; Rabelo Buzalaf, Marilia Afonso [4] ; Padilha, Pedro de Magalhaes [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Inst Biosci, Botucatu, SP - Brazil
[2] Univ Nebraska UNL, Lincoln, NE - USA
[3] Univ Brasilia UnB, Coll Planaltina, Brasilia, DF - Brazil
[4] Univ Sao Paulo, Bauru, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: BIOLOGICAL TRACE ELEMENT RESEARCH; v. 200, n. 4 SEP 2021.
Citações Web of Science: 0
Resumo

In recent decades, the scientific community has widely debated the contamination of fish in the Amazon region by mercury species. As the diet of riverside populations in the Amazon region is based mainly on fish, these populations are exposed to mercurial species that can cause serious and irreversible damage to their health. The risks of consuming fish exposed to mercurial species in the Amazon region have motivated toxicological investigations. However, the effect of mercurial species on protein and enzyme levels is still controversial. In this work, analytical and bioanalytical techniques Two-dimensional polyacrylamide gel electrophoresis {[}2D-PAGE] Graphite Furnace Atomic Absorption Spectrometry {[}GFAAS], and Mass Spectrometry in Sequence with Electrospray Ionization {[}ESI-MS/MS] were used to identify proteins associated with mercury (metal-binding protein) in muscle and liver tissues of the fish species Pinirampus pirinampu from the Madeira River, in the Brazilian Amazon. Enzymatic and lipid peroxidation analyses were also used to assess changes related to oxidative stress. Determinations of total mercury by GFAAS indicated higher concentrations in liver tissue (555 +/- 19.0 mu g kg(-1)) when compared to muscle tissue (60 +/- 2.0 mu g kg(-1)). The fractionation process of tissue proteomes by 2D-PAGE and subsequent mapping of mercury by GFAAS in the protein spots of the gels identified the presence of mercury in three spots of the liver tissue (concentrations in the range of 0.800 to 1.90 mg kg(-1)). The characterization of protein spots associated with mercury by ESI-MS/MS identified the enzymes triosephosphate isomerase A, adenylate kinase 2 mitochondrial, and glyceraldehyde-3-phosphate dehydrogenase as possible candidates for mercury exposure biomarkers. The muscle tissue did not show protein spots associated with mercury. Enzymatic activity decreased proportionally to the increase in mercury concentrations in the tissues. (AU)

Processo FAPESP: 16/19404-2 - Caracterização de biomarcadores de toxicidade de mercúrio em peixes da Bacia do Rio Madeira-Rondônia utilizando-se estratégias metaloproteômicas
Beneficiário:Pedro de Magalhães Padilha
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 14/02668-1 - Utilização de estratégias metaloproteômicas para avaliação de metalotioneínas em amostras de tecido hepático de peixes coletados no reservatório do Ahe Jirau - Bacia do Rio Madeira, RO, Brasil
Beneficiário:José Cavalcante Souza Vieira
Modalidade de apoio: Bolsas no Brasil - Doutorado