Biological Characterization of Polymeric Matrix and Graphene Oxide Biocomposites Filaments for Biomedical Implant Applications: A Preliminary Report

Autor(es):Mostrar menos -

Silva, Thamires Santos ^[1] ; Soares, Marcelo Melo ^[2] ; Oliveira Carreira, Ana Claudia ^[1] ; Schiavo Matias, Gustavo de Sa ^[1] ; Tegon, Carolina Coming ^[2] ; Massi, Marcos ^[2] ; Oliveira, Andressa de Aguiar ^[2] ; da Silva Junior, Leandro Norberto ^[1] ; Costa de Carvalho, Hianka Jasmyne ^[1] ; Dona Rodrigues Almeida, Gustavo Henrique ^[1] ; Araujo, Michelle Silva ^[1] ; Fratini, Paula ^[1] ; Miglino, Maria Angelica ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Surg, BR-05508270 Sao Paulo - Brazil

^[2] Univ Prebiteriana Mackenzie, Mackgraph Inst, Dept Mat Engn, BR-01302907 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: POLYMERS; v. 13, n. 19 OCT 2021.
• Citações Web of Science: 0

Resumo

Carbon nanostructures application, such as graphene (Gr) and graphene oxide (GO), provides suitable efforts for new material acquirement in biomedical areas. By aiming to combine the unique physicochemical properties of GO to Poly L-lactic acid (PLLA), PLLA-GO filaments were produced and characterized by X-ray diffraction (XRD). The in vivo biocompatibility of these nanocomposites was performed by subcutaneous and intramuscular implantation in adult Wistar rats. Evaluation of the implantation inflammatory response (21 days) and mesenchymal stem cells (MSCs) with PLLA-GO took place in culture for 7 days. Through XRD, new crystallographic planes were formed by mixing GO with PLLA (PLLA-GO). Using macroscopic analysis, GO implanted in the subcutaneous region showed particles' organization, forming a structure similar to a ribbon, without tissue invasion. Histologically, no tissue architecture changes were observed, and PLLA-GO cell adhesion was demonstrated by scanning electron microscopy (SEM). Finally, PLLA-GO nanocomposites showed promising results due to the in vivo biocompatibility test, which demonstrated effective integration and absence of inflammation after 21 days of implantation. These results indicate the future use of PLLA-GO nanocomposites as a new effort for tissue engineering (TE) application, although further analysis is required to evaluate their proliferative capacity and viability.</p> (AU)

• Processo FAPESP: 14/50844-3 - Matriz extracelular na saúde e matriz placentária na regeneração de tecidos
• Beneficiário: Maria Angelica Miglino
• Modalidade de apoio: Auxílio à Pesquisa - Temático