Nanozeolites as support for laccase immobilization: Application to mediated glycerol oxidation

Autor(es)

Miller, Alex Henrique ^{[1, 2]} ; de Vasconcellos, Adriano ^[2] ; Fielding, Alistair John ^[3] ; Nery, Jose Geraldo ^[2]

Afiliação do(s) autor(es)

^[1] Univ York, Dept Chem, York YO10 5DD, N Yorkshire - England

^[2] Sao Paulo State Univ UNESP, Inst Biosci Letters & Exact Sci IBILCE, Phys Dept, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil

^[3] Liverpool John Moores Univ, Ctr Nat Prod Discovery, Sch Pharm & Biomol Sci, James Parsons Bldg, Byrom St, Liverpool L3 3AF, Merseyside - England

• Tipo de documento: Artigo Científico
• Fonte: APPLIED CATALYSIS A-GENERAL; v. 626, SEP 25 2021.
• Citações Web of Science: 0

Resumo

This study investigated the use of nanozeolites as support for laccases from P. ostreatus (LPO), Aspergillus sp (LAsp) and A. bisporus (LAB) immobilization applied to 2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) mediated glycerol oxidation. Selected complexes led to up to 5% glycerol conversion, and interestingly, up to 100% selectivity to glyceraldehyde after 48 h. Free enzymes led to significantly higher yields (up to 82%) but lacked selectivity when tested under the same conditions. These findings suggest that laccases immobilized into nanozeolites are promising catalysts for the selective oxidation of glycerol. With the aim to understand the different behavior of free or immobilized enzymes, electron paramagnetic resonance (EPR) spectroscopy was applied. A significant shift of the T2 parallel copper hyperfine coupling constant was observed. This suggested a perturbation on the catalytic site after immobilization due to pH variation of the enzymatic microenvironments, thus influencing performance of laccase immobilized on nanozeolites. (AU)

• Processo FAPESP: 18/21483-3 - Investigação espectroscópica e eletroquímica da oxidação de glicerol Lacase/TEMPO-mediada
• Beneficiário: Alex Henrique Miller
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 16/24303-0 - Síntese e caracterização de novos biocatalisadores formados por complexos de nanozeólitas e enzimas lacases: suas aplicações em reações de oxidação do glicerol a compostos ácido tartrônico e ácido mesoxálico
• Beneficiário: Alex Henrique Miller
• Modalidade de apoio: Bolsas no Brasil - Doutorado