Vitamin C supplementation improves growth performance and caudal fin regeneration in zebrafish (Danio rerio)

Autor(es)

Pucci Figueiredo Carvalho, Pedro Luiz ^[1] ; Ventura Almeida, Pedro Henrique ^[1] ; Xavier, William dos Santos ^[1] ; Tiagua Vicente, Igor Simoes ^[1] ; Guimaraes, Matheus Gardim ^[1] ; Damasceno Rodrigues, Edgar Junio ^[1] ; Ito, Paulo Incane ^[1] ; Pezzato, Luiz Edivaldo ^[1] ; Barros, Margarida Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Juilo de Mesquita Filho, UNESP, Dept Breeding & Anim Nutr, Rua Prof Dr Walter Mauricio Correa S-N, BR-1861868 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: AQUACULTURE NUTRITION; v. 27, n. 1 NOV 2021.
• Citações Web of Science: 0

Resumo

This study aimed to evaluate the growth performance and fin regeneration rates of zebrafish fed with diets containing levels of vitamin C (Vit C). A basal practical diet was formulated to test six levels of Vit C supplementation (0, 75, 150, 300, 600 and 1,200 mg kg(-1)). A total of 504 juvenile zebrafish were distributed into thirty-six 8-L aquaria, constituting an experimental design with six treatments and six replicates. After 60 days of feeding, growth performance was evaluated. To analyse the regeneration rates of the caudal fin, 18 fish of each treatment were anesthetized and had part of their fins amputated. To determine the regenerated portion on Days 5, 10 and 15 postamputation, six fish of each treatment were subjected to image analysis. The average weight gain, specific growth rate, feed conversion ratio and final length were positively affected (p < .05) by Vit C supplementation. The diets with higher dietary concentrations of Vit C presented the best results regarding tissue regeneration. Based on the broken-line regression analysis, it is concluded that the Vit C nutritional requirement for the maximum growth and tissue regeneration of zebrafish juveniles fed with practical diets for 60 days were 188.7 and 897.4 mg kg(-1) diet, respectively. (AU)

• Processo FAPESP: 15/13798-6 - Efeitos da suplementação de vitamina C no desempenho produtivo e regeneração tecidual do zebrafish (Danio rerio)
• Beneficiário: Pedro Henrique Ventura de Almeida
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica