The effect of Haemonchus contortus and Trichostrongylus colubriforms infection on the ruminal microbiome of lambs

Autor(es)

Correa, Patricia Spoto ^{[1, 2]} ; Mendes, Lucas William ^[3] ; Lemos, Leandro Nascimento ^[4] ; Sampaio, Ana Claudia Koki ^[2] ; Issakowicz, Juliano ^[2] ; McManus, Concepta Margaret ^[5] ; Tsai, Siu Mui ^[3] ; Faciola, Antonio Pinheiro ^[1] ; Abdalla, Adibe Luiz ^[2] ; Louvandini, Helder ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 - USA

^[2] Univ Sao Paulo, Ctr Nucl Energy Agr, Lab Anim Nutr, BR-13400970 Piracicaba, SP - Brazil

^[3] Univ Sao Paulo, Ctr Nucl Energy Agr, Lab Mol Cell Biol, BR-13400970 Piracicaba, SP - Brazil

^[4] Natl Lab Sci Comp, Dept Bioinformat, BR-25651076 Petropolis, RJ - Brazil

^[5] Univ Brasilia, Inst Biol Sci, BR-70910900 Brasilia, DF - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Parasitology; v. 231, DEC 2021.
• Citações Web of Science: 0

Resumo

We evaluated Haemonchus contortus (HC) and Trichostrongylus colubriformis (TC) infection on the ruminal microbial community of Santa Ines lambs to better understand the pathophysiology of parasite infections and the interactions among gastrointestinal nematodes and gut resident microbiota. In this study, 18 six months of age lambs were maintained for 34 days in individual pens divided into three treatments that included animals infected with HC and TC, and control (infection-free). Haematological, ruminal parameter and microbial nitrogen absorbed by pune derivatives, as well as enteric methane emission (CH4), were analysed, and the rumen microbial taxonomic and functional profile assessed by shotgun metagenomics. The analysis showed that total protein, albumin, urea, and butyrate level were lower in animals infected by both parasites, while HC infection also decreased the haemoglobin level. Both infected groups (TC and HC) increased the enteric methane emission (CH4). TC and HC infections increased the diversity and richness of functional microbial genes. Most alterations in the rumen microbiome composition of infected groups are associated with the suppression of microbes involved in microbial homeostasis maintenance and expansion of the archaeal community in the infected animals. Infection led to an increased abundance of nitrogen, amino acid, protein, and energy metabolism genes. Overall, TC and HC infection increased the enteric methane emission, negatively affected taxon's responsible for maintenance de rumen homeostasis and modulated some important genes related to protein and energy metabolism. (AU)

• Processo FAPESP: 18/06191-6 - Transcriptoma em testículos de cordeiros oriundos de ovelhas alimentadas com caroço de algodão
• Beneficiário: Helder Louvandini
• Modalidade de apoio: Bolsas no Exterior - Pesquisa