A modular, reversible sealing, and reusable microfluidic device for drug screening

Autor(es)

Vit, Franciele Flores ^[1] ; Nunes, Rony ^[2] ; Wu, Yu Tzu ^[3] ; Soares, Marco Cesar Prado ^[3] ; Godoi, Naiara ^[1] ; Fujiwara, Eric ^[3] ; Carvalho, Hernandes F. ^[2] ; de la Torre, Lucimara Gaziola ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Chem Engn, Dept Mat & Bioproc Engn, Campinas - Brazil

^[2] Univ Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, Campinas - Brazil

^[3] Univ Estadual Campinas, Sch Mech Engn, Lab Photon Mat & Devices, Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Analytica Chimica Acta; v. 1185, NOV 15 2021.
• Citações Web of Science: 0

Resumo

Preclinical tests for evaluating potential drug candidates using conventional protocols can be exhaustive and high-cost processes. Microfluidic technologies that can speed up this process and allow fast screening of drugs are promising alternatives. This work presents the design, concept, and operational conditions of a simple, modular, and reversible sealing microdevice useful for drug screening. This microdevice allows for the operation of 4 parallel simultaneous conditions and can also generate a diffusive concentration gradient in sextuplicates. We used laminated polydimethylsiloxane (PDMSLAM) and glass as building materials as proof of concept. The PDMSLAM parts can be reused since they can be easily sterilized. We cultured MCF-7 (Michigan Cancer Foundation-7) breast cancer cells. Cells were exposed to a doxorubicin diffusive concentration gradient for 3 h. They were monitored by automated microscopy, and after data processing, it was possible to determine cell viability as a function of doxorubicin concentration. The reversible sealing enabled the recovery of the tested cells and image acquisition. Therefore, this microdevice is a promising tool for drug screening that allows assessing the cellular behavior in dynamic conditions and the recovery of cells for afterward processing and imaging. (C) 2021 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 17/20445-8 - Desenvolvimento de sensor de fibra óptica para monitoramento de reflectância e espalhamento de luz para microfluídica aplicada à biotecnologia
• Beneficiário: Marco César Prado Soares
• Modalidade de apoio: Bolsas no Brasil - Mestrado