Combining Coaxial Electrospinning and 3D Printing: Design of Biodegradable Bilayered Membranes with Dual Drug Delivery Capability for Periodontitis Treatment

Autor(es)

dos Santos, Danilo M. ^[1] ; de Annunzio, Sarah R. ^[2] ; Carmello, Juliana C. ^[3] ; Pavarina, Ana C. ^[3] ; Fontana, Carla R. ^[2] ; Correa, Daniel S. ^[1]

Afiliação do(s) autor(es)

^[1] Embrapa Instrumentacao, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos, SP - Brazil

^[2] UNESP Sao Paulo State Univ, Sch Pharmaceut Sci, Dept Clin Anal, BR-14801903 Araraquara, SP - Brazil

^[3] UNESP Sao Paulo State Univ, Sch Dent, Dept Dent Mat & Prosthodont, BR-14801903 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED BIO MATERIALS; DEC 2021.
• Citações Web of Science: 1

Resumo

Periodontitis is a chronic inflammatory disease that can lead to significant destruction of tooth-supporting tissues, compromising dental function and patient's health. Although the currently employed treatment approaches can limit the advance of the disease, the development of multifunctional and hierarchically structured materials is still in demand for achieving successful tissue regeneration. Here, we combine coaxial electrospinning and 3D printing techniques to prepare bilayered zein-based membranes as a potential dual drug delivery platform for periodontal tissue regeneration. A layer of core-sheath electrospun nanofibers consisting of poly(ethylene oxide) (PEO)/curcumin (Curt)/tetracycline hydrochloride (TH) as the core and zein/poly(epsilon-caprolactone)(PCL)/beta-glycerolphosphate (beta-GP) as the sheath was deposited over a 3D printed honeycomb PLA/zein/Curc platform in order to render a bilayered structure that can mimic the architecture of periodontal tissue. The physicochemical properties of engineered constructs as well as the release profiles of distinct drugs were mainly controlled by varying the concentration of zein (10, 20, 30%, w/w relative to dry PCL) on the sheath layer of nanofibers, which displayed average diameters ranging from 150 to 400 nm. In vitro experiments demonstrated that the bilayered constructs provided sustained release of distinct drugs over 8 days and exhibited biocompatibility toward human oral keratinocytes (Nok-si) (cell viability >80%) as well as antibacterial activity against distinct bacterial strains induding those of the red complex such as Porphyromonas gingivalis and Treponema denticola, which are recognized to elicit aggressive and chronic periodontitis. Our study reveals the potential of zein-based bilayered membranes as a dual drug delivery platform for periodontal tissue regeneration. (AU)

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