inking maximal shear rate and energy dissipation/circulation function in airlift bioreactor

Autor(es)

Esperanca, Mateus N. ^[1] ; Buffo, Mariane M. ^[2] ; Mendes, Caroline E. ^[3] ; Rodriguez, Guilherme Y. ^[4] ; Bettega, Rodrigo ^[2] ; Badino, Alberto C. ^[2] ; Cerri, Marcel O. ^[5]

Afiliação do(s) autor(es)

^[1] Fed Inst Educ Sci & Technol Sao Paulo, Campus Capivari, BR-13360000 Capivari, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Chem Engn, CP 676, BR-13565905 Sao Carlos, SP - Brazil

^[3] Fed Univ Rio Grande, Chem & Food Sch, Carreiros, RS - Brazil

^[4] Univ Fed Itajuba, Nat Resources Inst, Bioproc Engn Course, Itajuba, MG - Brazil

^[5] Sao Paulo State Univ, Fac Pharmaceut Sci, Dept Bioproc Engn & Biotechnol, BR-14801902 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Biochemical Engineering Journal; v. 178, JAN 2022.
• Citações Web of Science: 0

Resumo

The interest in pneumatic bioreactors is related to their good mass transfer capacity, as well as lower operating costs, due to the simple mechanical structure. Knowledge of the transport phenomena and hydrodynamics of bioreactors is important to enable definition of the best bioreactor model and operating conditions for a specific bioprocess, with the imposed shear being one of the most difficult to quantify. For stirred tanks, the fragmentation of microorganisms has been well correlated with a hydrodynamic parameter called the ``energy dissipation/circulation function{''} (EDCF). However, there have been no estimates of the EDCF for pneumatic bioreactors. The present work proposes a methodology to estimate the EDCF for different pneumatic bioreactors and operating conditions using two approaches: Computational Fluid Dynamics and, comparison of pellet fragmentation in conventional and pneumatic bioreactors. First, the volume of higher energy dissipation was estimated in 5 L pneumatic bioreactors, exhibiting values ranging from 57.9 to 106.5 cm(3). Subsequently, EDCF values were estimated, ranging from 0.71 to 9.1 kW.m(-3).s(-1) for pneumatic bioreactors. Finally, these values were validated by pellet fragmentation assays in stirred tank and pneumatic bioreactors under conditions of similar fragmentation, that resulted in similar EDCF values. (AU)

• Processo FAPESP: 18/11405-5 - Fermentação Alcoólica Extrativa em Biorreatores Pneumáticos com Arraste de Etanol por CO2 Integrada a Etapas de Recuperação
• Beneficiário: Alberto Colli Badino Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/08699-7 - Melhoria de desempenho em biorreator Airlift de circulação interna através de modificações geométricas no tubo interno
• Beneficiário: Marcel Otavio Cerri
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/23807-1 - Desenvolvimento e avaliação das condições de operação de biorreatores visando a produção de produtos biotecnológicos
• Beneficiário: Alberto Colli Badino Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/17756-8 - Avaliação das condições hidrodinâmicas, de cisalhamento e de transferência de oxigênio de diferentes modelos de biorreatores pneumáticos
• Beneficiário: Caroline Eliza Mendes
• Modalidade de apoio: Bolsas no Brasil - Doutorado