| Texto completo | |
| Autor(es): |
Mattiello, Lucia
;
Rutgers, Mark
;
Sua-Rojas, Maria Fernanda
;
Tavares, Rafael
;
Soares, Jose Sergio
;
Begcy, Kevin
;
Menossi, Marcelo
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN PLANT SCIENCE; v. 13, p. 18-pg., 2022-05-31. |
| Resumo | |
The prokaryote-derived Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/Cas mediated gene editing tools have revolutionized our ability to precisely manipulate specific genome sequences in plants and animals. The simplicity, precision, affordability, and robustness of this technology have allowed a myriad of genomes from a diverse group of plant species to be successfully edited. Even though CRISPR/Cas, base editing, and prime editing technologies have been rapidly adopted and implemented in plants, their editing efficiency rate and specificity varies greatly. In this review, we provide a critical overview of the recent advances in CRISPR/Cas9-derived technologies and their implications on enhancing editing efficiency. We highlight the major efforts of engineering Cas9, Cas12a, Cas12b, and Cas12f proteins aiming to improve their efficiencies. We also provide a perspective on the global future of agriculturally based products using DNA-free CRISPR/Cas techniques. The improvement of CRISPR-based technologies efficiency will enable the implementation of genome editing tools in a variety of crop plants, as well as accelerate progress in basic research and molecular breeding. (AU) | |
| Processo FAPESP: | 20/15230-5 - Centro de Pesquisa e Inovação de Gases de Efeito Estufa - RCG2I |
| Beneficiário: | Julio Romano Meneghini |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia |